直接涂片ABC-AP染色在急性白血病免疫分型诊断中的价值  

THE VALUE OF DIRECT SMEAR AND ABC-AP STAINING METHOD IN IMMUNOPHENOTYPING DIAGNOSIS OF ACUTE LEUKEMIAS

作　　者：赵利东[1] 尤世春 王明艳[2] 张桂敏[2] 杨建峰[1] 

机构地区：[1]连云港市第一人民医院血液科,江苏连云港222002 [2]鞍钢铁西医院检验科及血液科,辽宁鞍山114012

出　　处：《白血病．淋巴瘤》2004年第4期209-211,共3页Journal of Leukemia & Lymphoma

摘　　要：目的:探讨直接涂片ABC蛳AP染色在急性白血病免疫分型诊断中的价值。方法:对102例急性白血病患者的骨髓或外周血直接涂片,应用13种白细胞系列单克隆抗体,ABC免疫酶标法进行免疫学分型。结果:在73例ANLL中,纯髓系表型占90.4 %(66/73),粒单系特异性标记单抗表达率高低依次为CD+13 80.6%(58/72);CD+33 68.1 %(49/72);CD+15 55.6 %(40/72);29例ALL中,纯淋巴系表型为93.1%(27/29)。B细胞系特异性单抗标记表达率高低依次为CD+19 83.3 %(20/24);CD+22 70.8 %(17/24);CD+10 41.7 %(10/24)。T细胞系标记表达率高低依次为CD+5 和CD+7均为100 %(5/5);CD+2 80 %(4/5);CD+3 60 %(3/5)。102例急性白血病中,形态学分型正确归类者为96例,符合率为94.1 %(96/102)。结论:直接涂片ABC蛳AP染色,方法简便易行,结果可靠。Objective: To explore the value of direct smear and ABC- AP staining method in immunophenotyping diagnosis of acute leukemias(AL). Methods: The immunophenotyping of 102 cases with AL were detected by 13 leukon monoclonal antibodies with direct smear of peripheral blood or bone marrow and ABC- AP staining method. Results: The result of imunophenotyping showed the pure myeloid phenotype was 90.4 % and the order of myeloid lineage-specific marker expressin was as follows CD+13 80.6 %(58/72);CD+33 68.1 %(49/72);CD+15 55.6 %(40/72);In 29 cases with ALL, The pure lymphocytic phenotype was 93.1 %(27/29), the order of lymphoid lineage- specific marker expression was as follows CD+19 83.3 %(20/24);CD+22 70.8 %(17/24);CD+10 41.7 %(10/24)in B- cell, and CD+5 and CD+7 100 %(5/5);CD+2 80 %(4/5);CD+3 60 %(3/5)in T- cell. 96 cases with AL were correctly classified by morphological classification in 102 cases with AL. The coincidence rate was 94.1 % with imunophenotyping. Conclusion: The direct smear and ABC- AP staining method are simple and convenient ,also its results are reliable. Imunophenotyping has important clinic value for the differential diagnosis of ALLand ANLL and the diagnosis of rare AL.

关 键 词：直接涂片 ABC-AP染色 免疫分型 白血病 急性 

分 类 号：R733.72[医药卫生—肿瘤]