锁阳药材的定性鉴别方法研究  

Studies on the identification of Herba Cynomorii

作　　者：张思巨[1] 刘丽[1] 于江泳[1] 

机构地区：[1]中国中医研究院中药研究所,北京100700

出　　处：《中国药学杂志》2004年第8期579-581,共3页Chinese Pharmaceutical Journal

基　　金：国家重点科技攻关项目(99-929-01-24)

摘　　要：目的 建立锁阳药材的定性鉴别方法。方法 采用薄层色谱法对锁阳5种成分(+)-儿茶素、熊果酸、乙酰熊果酸、柑桔素、柑桔素-4'-O-吡喃葡萄糖苷进行定性鉴别;采用ELSD-HPLC方法对其醇溶物进行指纹图谱探索。结果 样品的TLC图谱斑点清晰,重复性好;样品指纹图谱相对保留时间的RSD小于0.5％。结论 本方法专属性强,为锁阳质量控制提供了可靠的定性鉴别方法。OBJECTIVE: To establish a method for the identification of Herba Cynomorii. METHODS: Five main components were identified by TLC and fingerprint of the alcoholic extract was investigated by ELSD-HPLC. RESULTS: TLC chromatograms of ten samples showed clear spots with good reproducibility. The RSD of relative retention time of common peaks in the fingerprints of six samples were less than 0.5%. CONCLUSION: The method can be used scientifically to evaluate the identity of Herba Cynomorii for quality control.

关 键 词：锁阳 中药材 定性鉴别 薄层色谱法 儿茶素 熊果酸 

分 类 号：R282.71[医药卫生—中药学]