促性腺激素作用下豫医无毛小鼠超排卵效果观察  

Study on PMSG-stimulated superovulation in Yuyi hairless mice

作　　者：杜春燕[1] 李洪涛[1] 史崇敏[1] 王纯耀[1] 杜献堂[1] 

机构地区：[1]郑州大学实验动物中心,郑州450052

出　　处：《郑州大学学报（医学版）》2004年第5期768-769,共2页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省自然科学基金资助项目 3 110 43 60 0

摘　　要：目的 :探讨近交系豫医无毛小鼠超排卵的最佳条件 ,提高超排卵效果。方法 :30只近交系豫医无毛小鼠随机分为 3组 ,分别用 5IU、8IU、10IU的孕马血清促性腺激素 (PMSG)和人绒毛膜促性腺激素 (HCG) ,间隔 4 8h注射 ,比较排出卵母细胞的数量。结果与结论 :腹腔间隔 4 8h各注射 8IU的PMSG和HCG超排卵效果最好 ;各剂量组右侧卵巢比左侧卵巢超排卵效果好 (P <0 .0 5 )。Aim: To find out the best way of superovulation in Yuyi hairless mice. Methods: A total of 30 mices were divided into 3 groups randomly.5 IU+5 IU,8 IU+8 IU or 10 IU+10 IU PMSG and HCG were used to stimulate superovulation in Yuyi hairless mice. After PMSG given on the first day,HCG was injected on the third day (48 h later). Ova were flushed out of the ovi-duct 18 h after administration of HCG.The ova's amount of each group was compared. Results and conclusion:The superovulation of the second group (8 IU+8 IU PMSG and HCG,48 h) was the best.The amount of right ova was more than that of the left by superovulation.

关 键 词：近交系 豫医无毛小鼠 超排卵 

分 类 号：R-332[医药卫生]