应用人类特异性引物扩增无毛小鼠SRY及AZF基因  

Amplification of SRY and AZF genes in hairless mouse by human specific primers

作　　者：王纯耀[1] 刘永波[2] 宋国英[2] 李晓文[2] 陈辉[2] 

机构地区：[1]郑州大学实验动物中心,郑州450052 [2]郑州大学细胞生物学与遗传学教研室,郑州450052

出　　处：《郑州大学学报（医学版）》2004年第5期769-772,共4页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省医学科技创新人才工程项目 2 0 0 10 90 7

摘　　要：目的 :了解人类SRY和AZF基因与雄性无毛小鼠生精基因的相关性。方法 :应用人类特异性引物扩增近交系无毛小鼠、昆明小鼠、DBA小鼠、HLC近交系小鼠的SRY和AZFa、AZFb、AZFc基因。结果 :4种小鼠均能扩增出SRY和AZFa、AZFb、AZFc基因 ,但小鼠的SRY和AZFb基因的PCR产物片段大小与人类的不一致。结论 :①无毛小鼠的SRY和AZFa、AZFb、AZFc基因与其他 3个品系小鼠的扩增产物片段大小无品系间差异。②雄性无毛小鼠繁殖率低的原因可能与生精相关基因无关。Aim:To explore the relationship of human sex-determing region Y (SRY) , azoospermia factor(AZF), and reproductive ability of hairless mouse. Methods:Using human specific primers, SRY, AZFa, AZFb, AZFc were used to amplify DNAs of hairless mouse, KunMing mouse, DBA mouse, and HLC mouse. Results: The four fragments were duplicated in all these four kinds of mouse, but fragment size of SRY and AZFb were different between mouse and human being. Conclusion: ①There are no distinct differences to fragment size of SRY, AZFa, AZFb, and AZFc between hairless mouse and all other three species mouse. ② The fact of lower reproduction rate of hairless mouse maybe has no any relationship to spermatogenesis.

关 键 词：无毛小鼠 睾丸决定基因 生精基因 繁殖 

分 类 号：R-332[医药卫生]