维A酸对体外培养的人牙周韧带细胞的生物学作用  

The biological effect of retinoic acid on cultured human periodontal ligament cells

作　　者：王燕[1] 汪说之[2] 杨丕山[1] 李纾[1] 

机构地区：[1]山东大学口腔医学院,山东济南250012 [2]武汉大学口腔医学院,湖北武汉430079

出　　处：《上海口腔医学》2004年第4期297-300,共4页Shanghai Journal of Stomatology

摘　　要：目的:探讨维A酸对体外培养的人牙周韧带细胞的生物学作用。方法:体外培养人牙周韧带细胞。分别以不同浓度的维A酸作用于细胞,在倒置显微镜下观察不同浓度的维A酸对细胞形态与排列的影响;并通过MTT法、酶动力学方法及考马斯亮蓝法检测维A酸对细胞增殖活性、碱性磷酸酶活性及蛋白质合成的影响。结果:维A酸能增强牙周韧带细胞的碱性磷酸酶活性,10-6mol／L浓度的维A酸对细胞的作用效果最明显(P<0.01);而对细胞形态与排列、增殖能力及总蛋白合成无影响。结论:维A酸可促进牙周韧带细胞向成骨样细胞方向分化。PURPOSE: To evaluate the biological effect of retinoic acid on cultured human periodontal ligament cells. METHODS: Human periodontal ligament cells were cultured and challenged by retinoic acid with different concentrations. The cell morphology was observed under phase-contrast microscope; the cell proliferation activity was examined using MTT; the alkaline phosphatase activity was measured by enzyme kinetics method; the total amount of protein was assayed at different times. RESULTS: Retinoic acid enhanced alkaline phosphatase activity and had no effect on morphology, proliferation and total protein synthesis of periodontal ligament cells. CONCLUSION: Retinoic acid improves periodontal ligament cells to differentiate into osteoblast like cells.

关 键 词：细胞培养 牙周韧带细胞 维A酸 碱性磷酸酶 

分 类 号：R781.4[医药卫生—口腔医学]