大鼠脑缺血再灌注后血清神经元特异性烯醇化酶变化及吲哚美辛的干预作用  

Changes of serum neuron-specific enolase levels and intervention effect of indomethacin in rats after cerebral ischemia-reperfusion

作　　者：白鹰[1] 佟风芝[1] 宋晶[1] 王卓尔[1] 宋瑜[1] 艾华[1] 

机构地区：[1]大连大学附属医院神经内科教研室,辽宁省大连市116021

出　　处：《中国临床康复》2004年第25期5283-5285,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:探讨大鼠脑缺血再灌注后血清神经元特异性烯醇化酶(neuro-specificenolase,NSE)水平和脑组织病理改变及吲哚美辛对脑缺血再灌注后的保护作用。方法:采用线栓法将SD大鼠制成大脑中动脉再灌注模型。第1部分将80只SD大鼠分成脑缺血2h再灌注后1,2,3,4,5d5个实验组。观测各组血清NSE,脑梗死灶体积及脑组织病理形态变化。第2部分探讨吲哚美辛对大鼠脑缺血再灌注后的保护作用,将30只SD大鼠分成对照组(脑缺血2h再灌注后3d),2个治疗组分别于再灌注前、后1h给药存活至再灌注后3d,观察组同前。结果:①脑缺血再灌注后脑梗死灶体积逐渐增大,第3天达高峰(P<0.05)。②血清NSE水平在脑缺血再灌注后逐渐升高,第3天达高峰(P<0.05),然后逐渐下降。③血清NSE水平与脑梗死灶体积呈正相关(r=0.92,P<0.001)。④病理检查可见神经组织缺血坏死改变于第3天最明显,并且在第3天时缺血脑组织中中性粒细胞浸润最明显。⑤两个治疗组脑梗死灶体积和血清NSE水平均明显小于对照组(P<0.001),脑组织病理形态改变轻微,中性粒细胞浸润不明显,再灌注前1h用药组效果更明显,但两个用药组之间无显著差异。结论:①血清NSE水平与脑梗死灶体积呈正相关,血清NSE可以作为脑缺血再灌注后神经元损害程度的标志物,还可以作为对脑缺血再灌注损伤保护作?AIM:To explore the serum neuron-specific enolase(NSE) levels,pathological changes of brain tissue in rats after cerebral ischemia-reperfusion and the protective effects of indomethacin on cerebral ischemia and reperfusion. METHODS:SD rat models of middle cerebral artery occlusion(MCAO) were established by using a thread occlusion.Firstly, 80 SD rats 2 hours after ischemia were divided into 1,2,3,4 and 5-day reperfusion groups;Secondly, the protective effect of indomethacin after cerebral ischemia-reperfusion was evaluated,and 30 SD rats were divided into control group(ischemia for 2 hours and reperfusion for 3 days) and 2 treatment groups(receiving administration before and 1 hour after reperfusion respectively and surviving to 3 days after reperfusion).The changes of serum NSE levels,cerebral infarction size and morphology of brain tissue were observed and detected in all the groups. RESULTS:①The cerebral infarction size increased gradually after ischemia-reperfusion,and reached its peak value on the 3rd day(P< 0.05).②Serum NSE levels increased gradually after ischemia-reperfusion,and reached its peak value on the 3rd day(P< 0.05),and then decreased gradually.③Serum NSE level had a significant positive correlation with cerebral infarction size(r=0.92,P< 0.001).④Pathological observation showed that cerebral ischemic and necrotic changes were most evidently on the 3rd day and polymorphonuclear leukocyte(PMN) infiltration was most obvious on the 3rd day of reperfusion.⑤Serum NSE and cerebral infarction size in the two treatment groups were obviously lower than those in the control group(P< 0.001).Fewer morphological changes in brain tissue and less PMN infiltration were found in the two treatment groups,and the effect were more obviously in the two treatment groups,but there was no significant difference between them. CONCLUSION:①Serum NSE level has a significant positive correlation with cerebral infarction size,suggesting that it can be used as a parameter both for the severity of neuron injury after

关 键 词：大鼠 脑缺血 再灌注损伤 血清 神经元特异性烯醇化酶 吲哚美辛 干预作用 

分 类 号：R743.31[医药卫生—神经病学与精神病学]