血管性痴呆大鼠海马区的长时程增强变化(英文)  

Changes of long-term potentiation of hippocampus in vascular dementia rats

作　　者：姚国恩[1] 王景周[1] 陈曼娥[1] 蒋晓江[1] 

机构地区：[1]解放军第三军医大学大坪医院野战外科研究所神经内科,重庆市400042

出　　处：《中国临床康复》2004年第25期5394-5396,共3页Chinese Journal of Clinical Rehabilitation

基　　金：重庆市科委资助项目(1999-2000)~~

摘　　要：背景:大鼠海马CA1区长时程增强(long-termpotentiation,LTP)作为学习记忆的细胞模型逐渐被认可,但其可能机制及其与病理改变关系明确报道尚较少。目的:观察四血管阻断大鼠海马CA1区LTP的变化,并探讨其可能机制。设计:随机对照的实验研究。地点、材料和干预:本实验在解放军第三军医大学大坪医院野战外科研究所动物实验中心完成。实验选用老龄健康Wistar大鼠60只。按随机数字表法分为对照组和模型组。每组分3个时相点:2周、4周、2个月,每时相点大鼠10只。用改良Pulsinelli's四血管阻断法建立血管性痴呆大鼠模型;采用电脑控制的穿梭箱系统检测大鼠学习记忆;离体海马脑片诱导的CA1区LTP检测大鼠学习记忆电生理改变;透射电镜观察大鼠海马CA1区的超微结构改变。主要观察指标:主动回避反应(AAR)、LTP检测,海马CA1区超微观察。结果:脑缺血组大鼠AAR在2周时较对照组显著下降(P<0.05),4周和2月时更加明显(P<0.01)。对照组海马脑片可明显诱出LTP波形,脑缺血组各时相点均几乎诱不出LTP,条件刺激前后fEPSP斜率变化的百分数与对照组比较差别明显(P<0.05);但各时相点间无明显差别。脑缺血组海马CA1区神经元细胞核固缩,线粒体肿胀、颗粒减少、电子密度增高,轴突脱髓鞘,次级溶酶体形成,高尔基复合体扩张空泡、神经毡空泡?BACKGROUND:Long term potentiation(LTP) of CA1 region in the hippocampus of rats has been widely accepted as the cell model of learning and memory. However,there are few reports on its mechanism and pathological changes.OBJECTIVE:To observe the changes of LTP in CA1 region of rat's hippocampus when blocking four blood vessels and explore the possible mechanism.DESIGN:Randomized case controlled study.SETTING,PARTICIPANTS and INTERVENTIONS:This experiment was completed in the Experimental Animal Center of Field Operation Surgery Research Institute,Third Military Medical University of Chinese PLA.60 Wistar rats were divided into control group and model group by random digits table method. Each group was divided into three subgroups by time phase which was 2 weeks,4 weeks and 2 months, with each of 10 rats.Vascular dementia was produced in rats using a modification of Pulsinelli's four-vessel occlusion model. Shuttle box system controlled by computer was used to test the learning and memory of rats.LTP of CA1 region induced by extracorporeal hippocampus slices was used to test the electricity changes of leaning and memory of rats.Ultrastructure of CA1 region of rat's hippocampus was observed by transmission electron microscope. MAIN OUTCOME MEASURES:Active avoidance reaction(AAR);LTP detection; ultramicro observation of CA1 region of hippocampus.RESULTS:Compared with control group, the AAR in model group declined more prominent than that of control group in the 2nd week(P< 0.05).It reduced much greater in the 4th week and 2nd months(P< 0.01).In the control group,the slices of hippocampus could obviously induce the LTP waveform while there was no LTP induced in all time phases in model groups. There were differences on the percentage changes of fEPSP slope before and after conditioned stimulus and between model groups and control groups(P< 0.05).However,there was no difference among different time phases.There were some chronically ischemic and anoxic changes could be seen in CA1 region of hippocampus among model group

关 键 词：血管性痴呆 大鼠 长时程增强 学习记忆 海马CA1区 

分 类 号：R749.13[医药卫生—神经病学与精神病学]