烹调油烟冷凝物对细胞的氧化损伤研究  

Study on oxidative damage effects of cooking oil fume conden sation on BEAS-2B cell

作　　者：张荣芳[1] 陈锋[1] 朱茂祥[2] 杨陡华[2] 

机构地区：[1]南华大学公共卫生学院,衡阳421001 [2]军事医学科学院

出　　处：《中国公共卫生》2004年第3期355-356,共2页Chinese Journal of Public Health

摘　　要：目的　通过测定细胞内和细胞上清液中活性氧 (reactiveoxygenspecies ,ROS)水平 ,以及细胞膜上ATP酶的含量 ,对烹调油烟冷凝物 (COFC)诱发永生化人支气管上皮细胞 (BEAS -2B)产生ROS及其对细胞膜损伤进行研究。方法　采用分光光度法检测细胞外活性氧 ,荧光标记细胞内活性氧及ATP酶。结果　在烹调油烟染毒的细胞内ROS的产生与ATP酶的活性降低有关。结论　COF引起细胞膜损伤时 ,ROS的大量产生可能是ATP酶活性降低的主要因素 ,Ca2 + -ATP酶及Mg2 +ObjectiveThe reactive oxygen species (ROS) production an d ATPase in human bronchial epithelial cell line induced by cooking oil fume co ndensation were studied through measuring extra celluar and intracellular ROS le vels including super oxide anions (O 2) and hydrogen peroxides (H 2O 2),and A TPase contents.MethodsSepectrometric method and fluorometric method wer e used to measure the ROS reduced by COFC.And the activities of ATPase assayed b y biochemical method.ResultsThe ROS correlated with the decrease of contents of ATPase.ConclusionMembrane ATPase activity was inhibited by ROS ,especially Ca\+\{2+\}-ATPase and Mg\+\{2+\}-ATPase.

关 键 词：烹调油烟 活性氧 ATP酶 BEAS-2B细胞 

分 类 号：R122.2[医药卫生—环境卫生学]