正常淋巴液对大鼠急性微循环障碍的干预作用  

Effect of lymph on acute microcirculatory disturbance of rats induced by dextran 500

作　　者：韩敏[1] 李俊杰[1] 张静[1] 牛春雨[1] 姜华[1] 赵自刚[1] 刘彦凯[1] 张学锋[1] 

机构地区：[1]河北北方学院病理生理教研室,河北张家口075000

出　　处：《中国急救医学》2004年第9期656-658,共3页Chinese Journal of Critical Care Medicine

摘　　要：目的　研究正常淋巴液对高分子右旋糖酐 (Dextran 5 0 0 )所致大鼠急性微循环障碍的干预作用。方法　Wistar大鼠 2 0只 ,均分为两组 ,静注 10 %Dextran 5 0 0 (1g/kg)复制急性微循环障碍模型。造模 6min后 ,颈静脉注射小量肠淋巴液 (模型对照组以等量生理盐水代替 ) ,应用活体显微电视录像技术 ,观察大鼠肠系膜微循环变化 ,同时记录颈总动脉血压及存活时间。结果　①静注 10 %Dextran 5 0 0可引起急性微循环障碍 ,表现为各级微血管口径缩窄、微血流速度变慢、伴有红细胞聚集、积分值显著高于造模前 (P <0 0 5～ 0 0 1) ;②淋巴液可减轻急性微循环障碍 ,其微血管口径缩窄、流态异常、积分值增高、红细胞聚集程度均较对照组为轻 (P <0 0 5～ 0 0 1) ;③模型组血压进行性下降 ,但淋巴液组可维持较高水平 (P <0 0 5 ) ;④淋巴液组的存活时间长于对照组 (P <0 0 5 )。结论　淋巴液能明显改善Dextran 5 0 0所致的急性微循环障碍 ,较好地延缓血压下降并延长存活时间。Objective To study the interference effect of lymph on acute disturbance of microcirculation in rats induced by dextran 500.Methods Twenty male Wistar rats were anesthetized by intramuscular injection of pentobarbital sodium(50 mg/kg·bw),and administrated intravenously dextran 500 (1 g/kg·bw)within 1 min,then we duplicate the acute disturbance of microcirculation animal models.Lymph was infused through cervical vein with one fifteenth of body blood volume in a constant speed after 6 min of making model in lymph treatment group,and the lymph was replaced by the equivalent volume of normal saline (NS)in model group.The mesentery near the lower ileum was spread on the observed window to investigate the internal diameter of mesenteric micro-vessels(A 1,A 2,V 1,V 2)and micro-blood flow condition by using a vital microscope with TV recorder before and after making model and after lymph or NS treatment.The analysis of blood flow condition by Tian’s multiple score method was used.The rats were connected the right common carotid artery with pressure transducer. Blood pressure was monitored continuously, and the survival time was recorded.Results ①Dextran 500 could lead to acute disturbance of microcirculation in rats.The diameter of mesenteric micro-vessels contracted(P<005～001),the micro-blood flow speed slowed(P<005)and the red cell aggregated(P<0.05～0.01)after making model in the two groups,but the two groups had no statistical difference before and after making model(P>005).②Lymph can markedly improved disturbance of microcirculation.The abnormality of the diameter of mesenteric micro-vessels, the micro-blood flow speed and the condition of the red cell aggregation in lymph treatment group was significantly slighter than those of control group(P<005～001).③Lymph can improve blood pressure.The blood pressure droped continuously in control group after making model,but it can be kept in a higher level in lymph treatment group(P<005).④The survival time of the rats in lymph treatment group was signific

关 键 词：淋巴液 微循环 肠系膜 血压 高分子右旋糖酐 

分 类 号：R363[医药卫生—病理学]