大鼠骨髓基质细胞体外诱导分化为内皮细胞实验研究  

Multiplication and differentiation into endothelial cells of bone marrow stromal cells of rats in vitro

作　　者：王春雪[1] 万虹[2] 历俊华[2] 王拥军[1] 毛淑静[3] 刘丽萍[1] 翟晶[2] 

机构地区：[1]首都医科大学附属北京天坛医院神经内科,北京市100050 [2]北京市神经外科研究所,北京市100050 [3]河北医科大学第二医院

出　　处：《中国康复理论与实践》2004年第8期458-460,i001,共4页Chinese Journal of Rehabilitation Theory and Practice

摘　　要：目的探讨骨髓基质细胞 (BMSC)向内皮细胞分化的可行性。方法无菌条件下获取BMSC ,实验组以 40 0ng/ml重组人粒细胞 巨噬细胞集落刺激因子 (rhGM CSF)为诱导剂 ,进行体外诱导分化 ;对照组用 164 0培养液培养。 8d后进行免疫组化染色进行特异性内皮细胞标志物鉴定。结果分化后的细胞在光镜下具有内皮细胞的形态学特征 ;免疫组化染色证实实验组诱导后的细胞为内皮细胞。结论BMSC在高浓度rhGM CSF作用下 。ObjectiveTo explore the feasibility of bone marrow stromal cells (BMSC) of rats differentiating into endothelial cells.MethodsBone marrow cells were obtained by an aseptic technique. Afterwards, the obtained cells were divided into two groups: cells in test group were propagated in 1640 medium with recombinant human GM CSF (rhGM CSF) (400ng/ml), and that in control group were propagated in medium with 1640 medium only. The differentiated cells were detected by specific immunology marker by immunocytochemistry and immunofluorescence at day 8.ResultsThe differentiated cells demonstrated the characters of endothelial cells under phase contrast microscopy. Cells of the test groups demonstrated specific characters by immunocytochemistry stain.ConclusionBone marrow stromal cells can multiply vigorously and differentiate into cells with endothelial cells characters in the medium with high concentration rhGM CSF.

关 键 词：重组人粒细胞-巨噬细胞集落刺激因子(rhGM—CSF) 骨髓基质细胞(BMSC) 内皮细胞 冯威勒不兰特因子 (vWF) 因子Ⅷ相关抗原(FⅧ-R Ag) 

分 类 号：R743.05[医药卫生—神经病学与精神病学]