外源性雌二醇对大鼠再生肝细胞转录活性的调节  

THE REGULATION OF EXOGENOUS ESTRADIOL IN TRANSCRIPTIONALACTIVITY OF REGENERATING HEPATOCYTES IN RATS

作　　者：宁黔冀[1] 李恩中[1] 翟心慧[1] 魏凯[1] 李超堃[1] 徐存拴 

机构地区：[1]河南师范大学生命科学学院,新乡453002

出　　处：《解剖学报》2004年第4期413-416,共4页Acta Anatomica Sinica

基　　金：河南省自然科学基金资助项目 ( 2 0 0 1180 0 0 0 9) ;河南省高校青年骨干教师资助计划 ( 2 0 0 1 95 )

摘　　要：目的　探讨雌激素对大鼠再生肝细胞转录活性的影响。　方法　放射免疫法测定血清中雌二醇(E2 )含量 ;核仁组成区相关嗜银蛋白 (AgNORs)染色法 ,测定AgNORs颗粒数。　结果　去卵巢后大鼠血清中E2 含量随去卵巢天数的增加而逐渐降低 ,至去卵巢后第 15d已测不出血清中有E2 存在 ;去卵巢大鼠部分肝切除 (PH)后0～ 2 4h ,外源E2 有促进肝细胞转录的作用 ,且AgNORs颗粒数的增加幅度与外源E2 剂量呈正相关 ;去卵巢大鼠PH后 2 4～ 36h ,外源E2 剂量越高 ,AgNORs颗粒数增加量越小 ,但均高于对照组。　结论　雌激素对再生肝细胞转录活性有调节作用。Objective To research the effect of exogenous estradiol(E-2) on transcriptional activity of regenerating hepatocytes in rats. Methods The E-2 levels in serum were measured using RIA and the numbers of argyrophil nucleolar organizer regions(AgNORs) were investigated. Results Rat serum E-2 level gradually declined after ovariectomy,and no E-2 could be measured after the 15th day.Exogenous E-2 could promote transcriptional activity of regenerating rat hepatocytes,particularly during the first 24h after PH,there was a dose-dependent increase in numbers of AgNORs 24-36h after PH,the exogenous E-2 cause a dose-dependent decrease in numbers of AgNORs,but all of the values were higher than that of control group.Conclusion E-2 can regulate the transcriptional activity of regenerating hepatocytes in rats.

关 键 词：雌二醇 再生肝细胞 核仁组成区相关嗜银蛋白 大鼠 

分 类 号：Q454[生物学—生理学] R335.4[医药卫生—人体生理学]