吸烟鼠脑血管内皮细胞间粘附分子1的表达在动脉粥样硬化形成中的作用  

Effect of Expression of Intercellular Adhesion Molecule-1 on Atherosclerosis in Cerebral Vascular Endothelial Cell of Cigarette Smoking Rat

作　　者：柳忠兰[1] 孙大勇[1] 冯昱[1] 拱忠影[1] 耿建红[1] 

机构地区：[1]中国医科大学附属第一医院神经内科,辽宁省沈阳市110001

出　　处：《中国动脉硬化杂志》2004年第4期392-394,共3页Chinese Journal of Arteriosclerosis

基　　金：卫生部科学研究基金 ( 98 1 196);辽宁省自然基金( 9810 5 0 0 3 0 3 )资助

摘　　要：为观察吸烟对大鼠脑血管内皮细胞超微结构、细胞间粘附分子 1表达的影响 ,建立大鼠吸烟动物模型 ,将 75只大鼠随机分为长期大量组、长期小量组、短期大量组、戒烟组和对照组 ,免疫组织化学和原位杂交结合显微图像分析系统检测大鼠脑血管内皮细胞细胞间粘附分子 1的表达 ;电镜观察内皮细胞超微结构的改变。结果发现 ,吸烟大鼠脑血管内皮细胞细胞间粘附分子 1表达增加 (P <0 .0 5 ) ,与吸烟量和时程存在剂量—效应关系 ;内皮细胞超微结构改变显著。戒烟鼠细胞间粘附分子 1表达下调 (P >0 .0 5 ) ,内皮细胞超微结构病理改变明显恢复。结果提示 ,吸烟致脑血管内皮细胞细胞间粘附分子 1表达上调可能是吸烟导致动脉粥样硬化的分子机制之一。Aim To investigate effect of cigarette smoking onmorphology and expression of intercellular adhesion molecule-1 (ICAM-1), ICAM-1 mRNA in vascular endothelial cells of rats' brains and to further explore influence of cigarette smoking on atherogenesis. Methods 75 male Wistar rats were randomly subgrouped into long-term massive, short-term massive, long-term trifle, anti-smoking and normal control group. Immunohistochemistry technique, in situ hybridization technique and pathologic image analyzing system were used to determine the expression of ICAM-1, ICAM-1 mRNA in rats' cerebral vascular endothelial cells. Results In contrast to normal control group, expression of ICAM-1, ICAM-1 mRNA in rats' cerebral vascular endothelial cells increased in smoking (103.7±3.9 vs 99.7±7.1 vs 92.6±5.2 vs 94.8±5.1 vs 84.2±4.7 for the mean gray value of ICAM-1mRNA staining in normal control, anti-smoking, short-term massive, long-term trifle, long-term massive group, repectively; P<0.05), and ICAM-1, ICAM-1 mRNA were relevant to the duration and dose of smoking (P<0.05). Expression of ICAM-1 and ICAM-1 mRNA decreased after anti-smoking, and there was no significant difference compared with normal control group (P>0.05). The structure of endothelial cells changed greatly in smoking, and partly recovered after anti-smoking. Conclusion The upregulation of expression of ICAM-1 and ICAM-1 mRNA might be one of the important molecular mechanisms during the process of atherosclerosis in cigarette smoking rats.

关 键 词：神经病学 吸烟在动脉粥样硬化形成中的影响 原位杂交 吸烟 脑动脉粥样硬化 内皮细胞 细胞间粘附分子1 

分 类 号：R741[医药卫生—神经病学与精神病学]