大鼠半乳糖性白内障晶状体中TGF-β_1基因的表达  

Expression of TGF-β_(1) gene in lens epithelial cells of rat with galactose-induced cataract

作　　者：马卫列[1] 欧钢卫[1] 葛正龙[1] 蔡善君[2] 曾小平[2] 

机构地区：[1]遵义医学院生化教研室,563003 [2]遵义医学院眼科教研室,563003

出　　处：《眼科研究》2004年第5期453-455,共3页Chinese Ophthalmic Research

基　　金：国家自然科学基金(39460 0 77);贵州省科委基金 [(93)30 76]资助

摘　　要：目的　研究转化生长因子 β1(TGF β1)基因在大鼠白内障发生过程中的表达。方法　给 3周龄大鼠单眼球后注射 2 0 %半乳糖生理盐水诱导白内障的发生 ,运用RT PCR方法测定在不同时相大鼠晶状体中TGF β1基因的表达。结果　半乳糖注射后晶状体上皮细胞TGF β1mRNA于 8h开始上升 ,2 4h达高峰 ,是正常对照的 4倍 ,2d后迅速下降 ,4d完全恢复到正常水平。结论　在白内障形成的早期 ,晶状体细胞中TGF β1呈高表达 ,提示TGFObjective To investigate the expression of transforming growth factor-β_(1) (TGF-β_(1)) gene in galactose-induced cataract in rats. Methods Cataract was induced by single-retrobulbar-injection of galactose in three-week-old rats.Expression of TGF-β_(1) at different cataract phases was determined by RT-PCR. Results TGF-β_(1)mRNA began to increase at 8 hours after injection of galactose and reached fourfold that of normal levels at 24 hours.TGF-β_(1)mRNA level decreased rapidly 2 days later and returned to the normal level after 4 days. Conclusion There is an overexpression of TGF-β_(1)mRNA in early stage of galactose-induced cataract,which may play an important role in the galactose-cataractgenesis.

关 键 词：大鼠 半乳糖 白内障 晶状体 TGF-Β1基因 表达 

分 类 号：R776[医药卫生—眼科]