中华硬蜱特异性抗原的定位  

Localization of the specific antigens in Ixodes sineasis

作　　者：刘志刚[1] 叶炳辉[2] 朱清仙[3] 高波[1] 

机构地区：[1]深圳大学生命科学学院,深圳518060 [2]南京医科大学基础医学院 [3]江西医学院

出　　处：《中国人兽共患病杂志》2004年第9期748-750,共3页Chinese Journal of Zoonoses

基　　金：国家自然科学基金资助项目 (No 3 9660 0 73 )

摘　　要：采用LSAB免疫酶组化染色技术 ,对中华硬蜱特异性抗原定位进行分析 ;用中华硬蜱反复叮咬后的动物血清 ,经LSAB法抗原片染色 ;结果显示 :中华硬蜱唾液腺和中肠上皮呈阳性反应 ,而在蜱的肌肉组织、卵巢组织、血腔等器官组织均显阴性反应 ,提示中华硬蜱唾液腺和中肠上皮为特异性抗原所在部位 ,为中华硬蜱进一步抗原纯化。By means of LSAB immunohistochemical staining,the specific antigen localization of Ixodex sinensis was performed with sera from the hosts infected by Ixodex sinensis The results showed that the specific antigens were found to be positve in salivary gland and midgut epithelium but negative in musle tissue.ovary,hemal cavity and other tissues.These results prove that the specific antigens of Ixodex sinensis are only localized in salivary gland and midgut epithelium And this study has therefore laid a theoretical basis for further antigen purification and vaccine research

关 键 词：中华硬蜱 特异性抗原 定位 

分 类 号：R384.4[医药卫生—医学寄生虫学]