按酵母基因优化的HPV6型L1蛋白在大肠杆菌中高效表达  

HPV Type 6 L1 Gene Optimized with Yeast Preferred Codons Can Be Expressed Efficiently in E.coli

作　　者：李平川[1] 张晓光[1] 周玲[1] 曾毅[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所肿瘤病毒室,北京100052

出　　处：《病毒学报》2004年第3期266-269,共4页Chinese Journal of Virology

基　　金：国家高技术"863"计划资助项目(2001AA215221)

摘　　要：Human papillomavirus 6(HPV6) causes a common sexually transmitted disease,the genital warts.L1-capsid protein is a highly promising preventive vaccine that has entered phase Ⅱ clinical trial.But the existing methodologies for producing L1-capsid protein in insect cells is expensive for use in developing countries.In order to get larger amount of L1 protein in E.coli economically,we rebuilt whole L1 gene with preferred codons for yeast P.pastoris,abolished A-T rich regions,subcloned the modified L1 gene (mL1) into pTXB1 and induced expression by IPTG.SDS-PAGE and Western blot showed that mL1-6×His fused proteins could be overexpressed in BL21(DE3).This success will facilitate the HPV vaccine development and structure-function study.Human papillomavirus 6(HPV6) causes a common sexually transmitted disease,the genital warts.L1-capsid protein is a highly promising preventive vaccine that has entered phase Ⅱ clinical trial.But the existing methodologies for producing L1-capsid protein in insect cells is expensive for use in developing countries.In order to get larger amount of L1 protein in E.coli economically,we rebuilt whole L1 gene with preferred codons for yeast P.pastoris,abolished A-T rich regions,subcloned the modified L1 gene (mL1) into pTXB1 and induced expression by IPTG.SDS-PAGE and Western blot showed that mL1-6×His fused proteins could be overexpressed in BL21(DE3).This success will facilitate the HPV vaccine development and structure-function study.

关 键 词：人乳头瘤病毒6型 L1蛋白 酵母 基因优化 表达 大肠杆菌 

分 类 号：R373[医药卫生—病原生物学] Q78[医药卫生—基础医学]