普通野生稻东乡群体等位酶水平的遗传多样性研究  

Study on Genetic Diversity of Dongxiang Wild Rice (Oryza rufipogon Griff.) -Allozyme

作　　者：李桂花[1] 黎国喜[2] 黄英金[3] 陈大洲[4] 张衍荣[1] 曹健[1] 

机构地区：[1]广东省农业科学院蔬菜研究所,广东广州510640 [2]华南农业大学农学院作物生产系,广东广州510642 [3]江西农业大学农学院,江西南昌330045 [4]江西省农业科学院水稻研究所,江西南昌330002

出　　处：《作物学报》2004年第9期927-931,共5页Acta Agronomica Sinica

基　　金：国家自然科学基金 ( 3 99690 0 1)资助

摘　　要：利用普通野生稻东乡群体苗期幼叶 ,采用聚丙烯酰胺凝胶电泳技术 ,对采自异位种质圃内 9个小群体的材料进行研究。选取 12种酶系统进行等位酶分析 ,共获得 2 1个等位酶位点。对这 2 1个位点等位基因的统计分析表明 :普通野生稻东乡群体多态位点的百分数为P =2 3 3% ,等位基因平均数为A =1 16 6 ,观察杂合度为Ho=0 14 8,预期杂合度为He=0 0 89,固定指数F =- 0 6 6 0 ,基因分化系数GST=0 0 15。以上数据表明 :普通野生稻东乡群体偏离Hardy Weinberg平衡 ,小群间的遗传分化很小 ,遗传多样性主要存在于小群体内的单株间。Allozyme diversity of the Dongxiang wild rice(Oryza rufipogon Griff.) was detected by vertical slab polyacryla- mide gel electrophoresis. Twelve enzymes including twenty-one loci were assessed for nine subpopulations. The percentage of polymorphic loci(P), mean observed heterozygosity (H o) and mean expected heterozygosity (H e) were 23.3%, 0.148,0.089 respectively. The coefficient of gene differentiation was 0.015 and the F value of deviation from Hardy-Weinberg expectation was -0.660. The genetic diversity among subpopulation was low and most variation(98.5%) were within subpopulation.

关 键 词：普通野生稻 东乡 等位酶 小群体 遗传多样性 

分 类 号：S511.9[农业科学—作物学]