New serum biomarkers for detection of HBV-induced liver cirrhosis using SELDI protein chip technology  

作　　者：Xiao-DongZhu Wei-HuaZhang Cheng-LinLi YangXu Wei-JiangLiang PoTien 

机构地区：[1]DepartmentofMolecularVirology,InstituteofMicrobiology,Chinese-AcademyofSciences,Beijing100080,China [2]CiphergenBiosystems,Inc.,Beijing,China [3]DepartmentofPathology,BeijingYou'anHospital,Beijing100054,China

出　　处：《World Journal of Gastroenterology》2004年第16期2327-2329,共3页世界胃肠病学杂志（英文版）

基　　金：Supported by the Major State Basic Research Development Program of China(973 Program),No.2001CB510001

摘　　要：AIM: To find new serum biomarkers for liver cirrhosis (LC) in chronic carriers of hepatitis B virus (HBV).METHODS: Surface enhanced laser desorption/ionization time-of-flight (SELDI-TOF) mass spectrometry was used to discover biomarkers for differentiating HBV induced LC from non-cirrhotic cohorts. A training population of 25 patients with HBV-induced LC, 20 patients with HCC, and 25 closely age-matched healthy men, was studied.RESULTS: Two biomarkers with Mr 7 772 and 3 933 were detected in sera of non-cirrhotic cohorts, but not in patients with HBV-induced LC. A sensitivity of 80% for all LC patients,a specificity of 81.8% for all non-cirrhotic cohorts and a positive predictive value of 75% for the study population were obtained.CONCLUSION: These two serum biomarkers for HBV-induced LC might be used for diagnosis and assessment of disease progression.AIM:To find new serum biomarkers for liver cirrhosis (LC) in chronic carriers of hepatitis B virus (HBV). METHODS:Surface enhanced laser desorption/ionization time-of-flight (SELDI-TOF) mass spectrometry was used to discover biomarkers for differentiating HBV induced LC from non-cirrhotic cohorts.A training population of 25 patients with HBV-induced LC,20 patients with HCC,and 25 closely age-matched healthy men,was studied. RESULTS:Two biomarkers with M_r 7 772 and 3 933 were detected in sera of non-cirrhotic cohorts,but not in patients with HBV-induced LC.A sensitivity of 80% for all LC patients, a specificity of 81.8% for all non-cirrhotic cohorts and a positive predictive value of 75% for the study population were obtained. CONCLUSION:These two serum biomarkers for HBV- induced LC might be used for diagnosis and assessment of disease progression.

关 键 词：免疫血清 HBV SELDI 肝硬化 蛋白质碎片工艺 肝脏疾病 

分 类 号：R575.2[医药卫生—消化系统]