植物相关人肿瘤抗原在乳腺癌中表达的研究  

Expression of a plant associated human cancer antigen in breast cancer

作　　者：傅军[1] 田海梅[1] 曲平[1] 李茉[1] 郑新雯 郑振海 张伟[1] 

机构地区：[1]中国医学科学院中国协和医科大学肿瘤研究所肿瘤医院肿瘤生物学检测中心,北京100021 [2]河北省灵寿郑氏生物癌研究所

出　　处：《中华肿瘤杂志》2004年第7期403-405,共3页Chinese Journal of Oncology

摘　　要：目的　检测一种植物相关人肿瘤抗原在乳腺癌、乳腺良性病变和正常乳腺组织中的表达 ,并探讨其临床意义。方法　采用S P免疫组织化学方法 ,检测该抗原在 5例正常乳腺组织、2 0例乳腺纤维腺瘤和 136例原发性乳腺癌中的表达。结果　植物相关人肿瘤抗原在乳腺正常组织中无表达 ;在乳腺纤维腺瘤中 ,表达阳性率为 2 0 .0 % ,且表达强度较低 ;在乳腺癌组织中 ,该抗原阳性表达率为 85 .3% ,与前两者相比 ,差异有极显著性 (P <0 .0 1) ,其表达水平与乳腺癌的组织学分级 (P <0 .0 5 )、浸润 (P <0 .0 1)和复发 (P <0 .0 5 )密切相关。结论　植物相关人肿瘤抗原在乳腺癌的发生发展过程中可能起重要作用 ,是一种具有潜在乳腺癌诊断价值的肿瘤标志物。Objective To study the expression of a glycoprotein of plant orig in in normal,benign and malignant breast tissues. Methods Expression of a pla nt glycoprotein was examined in 5 samples of normal breast tissues,20 fibro-ad e noma and 136 breast cancer by SABC immunohistochemical staining and the results were analyzed by SPSS statistics software. Results No positive staining was detecte d in normal breast tissues (0/5). Weak staining was observed in 4 of 20 (20.0%) breast fibro-adenoma. Positive staining was demonstrated in 116 out of 136 (85. 3 %) breast cancer specimens. The differences were statistically significant. The expression of plant-associated human cancer antigen was related to pathological grade ( P <0.05),tissue invasiveness ( P < 0.01) and recurrence ( P <0 .05),but not to patients′ age,tumor size and c-erbB-2 expression. Conclusion The plant glycop rotein studied may be a human cancer-associated antigen which might be a potent ial marker of breast cancer.

关 键 词：植物 肿瘤抗原 乳腺癌 免疫组织化学 基因表达 乳腺组织 

分 类 号：R737.9[医药卫生—肿瘤]