中国汉族男性CYP17基因多态性与前列腺癌发生危险性的关系  

Association between the polymorphism of CYP17 gene and risk of prostate cancer in Chinese men

作　　者：王军起[1,3] 古力米热 李鸿伟[1] 刘建河[1] 佟明[1] 艾军魁[1] 袁亦铭[1] 辛殿祺[1] 李鸣[1] 那彦群[1] 

机构地区：[1]北京大学第一医院,北京大学泌尿外科研究所,现在100034 [2]乌鲁木齐市友谊医院 [3]徐州医学院附属医院泌尿外科

出　　处：《中华泌尿外科杂志》2004年第8期512-515,共4页Chinese Journal of Urology

基　　金：国家自然科学基金 ( 3 0 0 70 75 5 )

摘　　要：目的　探讨CYP1 7基因多态性与前列腺癌发生危险性的关系。　方法　提取 1 2 5例前列腺癌患者和 1 5 8例对照组的血DNA标本 ,设计引物通过PCR技术扩增出包括基因多态位点的片段 ,用限制性内切酶MspA1Ⅰ进行酶切 ,产物在 2 %琼脂糖凝胶上电泳 ,确定出CYP1 7基因的 3种基因型 ,即A1 /A1、A1 /A2、A2 /A2 ,并经测序证实。　结果　基因型A1 /A2、A2 /A2频数与A1 /A1基因型进行比较 ,A1 /A2和A2 /A2在前列腺癌中的频数与对照组相比 ,其OR值分别为 1 .1 9和 1 .2 8,P值分别为 0 .5 7和 0 .4 5 ;但年龄 >6 9岁的病例中 ,其OR值分别为 3.97和 3.2 1 ,P值分别为 0 .0 0 7和 0 .0 2 3;对照组的血fPSA、tPSA水平 3组间差异无显著性意义。　结论　A1 /A2、A2 /A2基因型未增加汉族人群前列腺癌发生的危险性 ,基因型间血PSA水平差异无显著性支持这一观点。年龄 >6 9岁的人群中 ,A1 /A2、A2 /A2基因型显著增加前列腺癌发生的危险性 ,提示CYP1Objective To investigate the association between the polymorphism of CYP17 gene and the risk of prostate cancer. Methods A case control study including 125 prostate cancer patients and 158 age matched controls was conducted.The polymorphism was investigated by PCR using DNA from peripheral blood lymphocytes. The transition (T→C) in the risk allele (A2) produced a new recognition site for the restriction enzyme MspA1.Three genotypes of CYP17 gene (A1/A1,A1/A2,A2/A2) were determined and confirmed by sequencing. Results The frequency of the A2 allele was similar in the cases and controls.Compared with men with A1/A1 genotype, the odds ratios were 1.19 and 1.28 for the A1/A2 and A2/A2 genotypes,respectively.However,stratification by age of prostate cancer cases revealed that among prostate cancer cases older than 69 years,the odds ratio was 3.97 and 3.21 for the A1/A2 and A2/A2 genotypes ( P =0.007,0.023,respectively).Meanwhile,comparison among 3 subgroups (division by genotypes) of controls,the fPSA and tPSA levels were not significantly different. Conclusions These results indicate that A2 allele does not increase the risk of prostate cancer in Chinese. But in men older than 69 years,A1/A2 and A2/A2 genotypes predict susceptibility to prostate cancer. It is suggested that CYP17 gene may be associated with the morbidity of prostate cancer in elderly male population.

关 键 词：中国 汉族 男性 CYPl7基因 基因多态性 前列腺癌 危险性 肿瘤 

分 类 号：R737.25[医药卫生—肿瘤]