复方中药紫龙金对前列腺癌的体外作用  

In vitro effects of compound Chinese herbal medicine Zilongjin on prostate cancer cell lines

作　　者：李学松[1] 梁云燕 王代树 谢贤和[3] 师长进[1] 陈捷[1] 周利群[1] 薛兆英[1] 那彦群[1] 郭应禄[1] 

机构地区：[1]北京大学第一医院北京大学泌尿外科研究所,100034 [2]北京大学肿瘤医院北京肿瘤防治研究所 [3]福建医科大学协和临床医学院

出　　处：《中华泌尿外科杂志》2004年第8期516-520,共5页Chinese Journal of Urology

基　　金：国家自然科学基金 ( 3 9480 0 2 6)

摘　　要：目的　探讨复方中药紫龙金对前列腺癌的体外作用机理。　方法　应用MTT法、软琼脂集落生长实验、流式细胞术和显微荧光术测定紫龙金对LNCaP、DU 1 4 5和PC 3细胞的增殖抑制、集落生长抑制、周期阻滞和诱导凋亡作用 ;应用RT PCR方法检测对前列腺特异性抗原基因PSA和雄激素受体基因AR、凋亡相关基因Bcl 2和Bax以及抑癌基因p1 6表达的影响。　结果　紫龙金对 3种前列腺癌细胞系均具有剂量依赖性增殖抑制和G0 /G1 期阻滞作用 ,作用 72h时 3种细胞IC50 分别为 0 .79、0 .4 2和 0 .5 2mg/ml;对LNCaP和DU 1 4 5细胞具有诱导凋亡作用 ,可以显著抑制DU 1 4 5细胞的集落生长 ;紫龙金可以下调LNCaP细胞PSA、AR和Bcl 2表达 ,下调DU 1 4 5细胞Bcl 2 ,上调Bax和P1 6基因表达。　结论　紫龙金可以通过抑制前列腺癌细胞增殖、抑制集落生长、G0 /G1 期阻滞、诱导凋亡、调节PSA、AR、Bcl 2、Bax和p1Objective To investigate the in vitro effects of compound Chinese herbal medicine Zilongjin on prostate cancer cell lines. Methods MTT assay,flow cytometry, colony on soft agar,and fluorescence microscopy were used to observe the effects of Zilongjin on anti proliferation,inhibition of clonal growth,cell cycle arrest and apoptosis induction.RT PCR method was used to study the regulative effect of Zilongjin on prostate marker genes PSA,AR,apoptosis related genes Bcl 2,Bax and tumor suppressing gene p16. Results Zilongjin caused notable anti proliferative effect,and induced G 0/G 1 phase arrest dose dependently on LNCaP,DU 145 and PC 3 cell lines.Inhibition of cell growth by 50% (IC 50 ) was observed with Zilongjin at concentrations of 0.79,0.42 and 0.52 mg/ml,respectively.Apoptosis induction was seen in LNCaP and DU 145 cell lines.Zilongjin also caused inhibition of clonal growth of DU 145 cell line.In addition,Zilongjin down regulated the expression of PSA,AR and Bcl 2 genes in LNCaP cell line;and down regulated Bcl 2,up regulated Bax and p16 genes expression in DU 145 cell line. Conclusions It is demonstrated that Zilongjin has antitumor action through anti proliferation,inhibition of clonal growth,G 0/G 1 phase arrest,apoptosis induction and regulating the expression of PSA,AR,Bcl 2,Bax and p16 genes.

关 键 词：复方中药 紫龙金 前列腺癌 肿瘤 植物制剂 

分 类 号：R737.25[医药卫生—肿瘤] R282.71[医药卫生—临床医学]