透射电镜观察缺血再灌注对大鼠心脏胶原的影响  

Observation on collagen of myocardial interstitium in rats by transmission electron microscope

作　　者：王琦[1] 郭志坤[1] 韩金珠[1] 

机构地区：[1]新乡医学院组织学与胚胎学教研室,新乡453003

出　　处：《解剖学杂志》2004年第4期366-369,共4页Chinese Journal of Anatomy

摘　　要：目的 :探讨正常大鼠心肌间质胶原纤维的形态及缺血、缺血—再灌注对其的影响。方法 :成年大鼠随机分成对照组、缺血组、缺血—再灌注组。夹闭左冠状动脉旋支制备缺血模型。在左冠状动脉旋支分布的左室前壁取心肌 ,透射电镜观察心肌间质胶原纤维。结果 :对照组胶原纤维含量丰富 ;缺血组胶原纤维发生了形态改变 ;缺血—再灌注组胶原纤维数量明显减少。结论 :缺血使心肌间质胶原纤维破坏 ,再灌注使其破坏加重。心肌间质胶原的破坏是影响心功能恢复的原因之一。Objective: To investigate the morphology of the myocardial collagen in rat heart after ischemia and ischemia-reperfusion. Methods: Mature rats were randomly divided into control group, ischemic group and ischemia-reperfusion group. Ischemic model was prepared by occluding circumflex branch of coronary artery. Myocardial block was cut from anterior wall of left ventricle which distributed in circumflex branch of coronary artery, and the collagen of myocardial interstitium was observed by transmission electron microscope. Results: Myocardial collagen fibers were abundant in control group, and morphological changes of myocardial collagen fibers occurred in ischemic group,The amount of collagen fibers decreased evidently in ischemia-reperfusion group. Conclusion: Myocardial collagen fiber is damaged in ischemic heart and seriously damaged in ischemia-reperfusion heart. Damaged myocardial collagen is one of the factors disturbing recovery of cardiac function.

关 键 词：透射电镜 缺血再灌注 大鼠 心脏胶原 心肌缺血 

分 类 号：R361[医药卫生—病理学]