肿瘤发展过程中类胡萝卜素、卟啉代谢的模拟及其荧光分析  

Fluorescence Study by Simulating the Metabolizability of Carotenoid and Porphyrin during Cancer Development

作　　者：于佳[1] 孟继武[1] 李颖[1] 马君[1] 郑荣儿[1] 

机构地区：[1]中国海洋大学光学光电子实验室,山东青岛266071

出　　处：《光谱学与光谱分析》2004年第8期981-983,共3页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金 (30 0 70 72 8)资助

摘　　要：通过肿瘤发展过程中类胡萝卜素、卟啉代谢的模拟及其荧光分析 ,研究了类胡萝卜素对血清荧光法癌诊断的影响。采取肘静脉血制取血清 ,采用光致发光的方法研究了血清中的发光中心 ,模拟癌发展过程中类胡萝卜素和卟啉的代谢水平对血清荧光癌诊断方法的影响。血清中主要的发光是来自具有共轭基团的血清蛋白、类胡萝卜素和卟啉的电子跃迁。随着血清中类胡萝卜素含量的增加 ,5 2 0nm荧光峰强度提高 ,6 14nm附近的波谷亦升高 ,诊断I值 (荧光强度的比值 ,见引言中公式 ( 1) )The purpose of the present work was to investigate the effects of carotenoid on the spectroscopic examination of human blood serum by simulating the metabolizability of the carotenoid and porphyrin during the development of cancer and by studying the fluorescence of them. Blood serum was prepared with blood sample from elbow vein and the luminescence center of blood serum was studied using photo induced-luminescence techniques. The major factors of blood serum fluorescence were electron transitions in serological protein, carotenoid and porphyrin molecules with conjugated groups.. The intensity of the fluorescence spectrum at 530 nm was enhanced while the minimum intensity at 614 nm was also enhanced with the content of carotenoid increasing, thus the value of diagnostic symbol I decreased, namely the veracity of cancer examination decreased.

关 键 词：肿瘤 类胡萝卜素 卟啉 模拟 荧光分析 血清荧光法 诊断 

分 类 号：R730.4[医药卫生—肿瘤]