肿瘤组织傅里叶变换红外光谱诊断方法的比较研究  

A Comparative Study of Malignant Tissue Diagnosis Using ATR and Microscopy FTIR Spectroscopy

作　　者：任予[1] 徐怡庄[1] 赵莹[2] 杨丽敏[1] 李庆波[1] 张元福[1] 翁诗甫[1] 石景森[3] 徐端夫[2] 吴瑾光[1] 

机构地区：[1]北京大学化学与分子工程学院 [2]中国科学院化学研究所,北京100080 [3]西安交通大学第一医院,陕西西安710061

出　　处：《光谱学与光谱分析》2004年第8期930-932,共3页Spectroscopy and Spectral Analysis

基　　金：国家重点基础发展规划 (2 0 0 2CCA0 1 90 0);国家自然科学基金 (30 371 60 4 ;50 2 0 30 0 1 );教育部博士点基金资助项目

摘　　要：在前期的肿瘤组织红外光谱诊断方法研究中 ,采用水平衰减全反射 (ATR)红外光谱诊断法在多种肿瘤组织样品中取得了与病理诊断很好符合的实验结果 ;针对文献报道中多采用的显微红外光谱法 ,文章对同一肿瘤组织样品应用两种方法采集红外光谱 ,比较测量条件及光谱诊断结果 ,研究结果表明ATR法主要测量了整块组织的光谱信息 ,避免了组织结构不均一 ,微区采样扫描取点少对光谱诊断结果的影响 ,获得的光谱可以为临床应用提供判断依据。A comparative FTIR study was undertaken between ATR (attenuated total reflection) and infrared microsoopy on their application to measuring the same tissue for cancer diagnosis. The measurements showed that the coincidence of pathological diagnosis with ATR is higher than that of microscopy FTIR method. The result indicated that the measured area of tumor tissue is critical. The area for microscopy measurement is similar to250 mum(2), it reflects the heterogeneity of the tissue. However, the ATR measurement provides more information of malignant tissue for the measured area is 1.9 x 10(5) times than microscopy. Therefore, ATR exhibit the high accuracy in cancer detection for clinical application.

关 键 词：肿瘤组织 傅里叶变换红外光谱 诊断 显微红外法 衰减全反射法 

分 类 号：R730.4[医药卫生—肿瘤]