木瓜总苷抑制小鼠接触性超敏反应及对其胸腺T淋巴细胞亚型的调节作用  

Glucosides of Chaenomeles speciosa suppressed contact hypersensitivity response via modulating the thymus T lymphocytes subsets in mice

作　　者：郑咏秋[1] 魏伟[1] 戴敏[1] 汪倪萍[1] 

机构地区：[1]安徽医科大学临床药理研究所,安徽合肥230032

出　　处：《中国药理学通报》2004年第9期1016-1020,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目;No 3 0 2 7160 6;安徽省自然科学基金资助项目;No 0 0 0 44 411;安徽省"十五"科技重大专项资金资助项目 ;No 0 180 3 0 2 6;合肥市重点科技项目资助项目;No 2 0 0 14 814

摘　　要：目的　探讨木瓜总苷对小鼠接触性超敏反应 (CHS)的影响及部分机制。方法　采用了 2 ,4 二硝基氟苯 (DNFB)诱导小鼠CHS模型 ,采用流式细胞术检测小鼠胸腺T淋巴细胞亚型 ,MTT法检测脾脏T淋巴细胞增殖 ,ConA诱导的胸腺T淋巴细胞培养上清中TGF β1和IL 4水平检测采用ELISA法 ,IL 2活性检测采用活化的小鼠脾细胞MTT比色法。结果　于初次致敏前 5d灌胃木瓜总苷 6 0、12 0、2 4 0mg·kg-1和阳性对照药阿克他利 12 0mg·kg-1,连续给药 10d。木瓜总苷 2 4 0mg·kg-1可降低CHS小鼠脾指数和胸腺指数、木瓜总苷 6 0、12 0、2 4 0mg·kg-1均可降低CHS小鼠耳肿胀度、抑制ConA诱导的脾T淋巴细胞过度增殖 ;木瓜总苷 2 4 0mg·kg-1可降低CHS小鼠胸腺异常增高的CD4 + CD8+ 亚型T淋巴细胞比例、恢复降低的CD4 + CD8-亚型T淋巴细胞比例 ;木瓜总苷 6 0、2 4 0mg·kg-1可恢复CHS小鼠低下的CD4 -CD8-亚型T淋巴细胞比例。同时 ,木瓜总苷还可有效抑制ConA诱导的CHS小鼠胸腺T淋巴细胞培养上清液中的TGF β1和IL 2水平 ,并对同一培养上清液中的IL 4水平也有提高作用。结论　木瓜总苷对小鼠CHS有明显抑制作用 ;可有效抑制过度的脾T淋巴细胞增殖 。Aim To investigate the effects and mechanisms of glucosides of chaenomeles speciosa (GCS) in mice with contact hypersensitivity (CHS) response. Methods CHS model in mice induced by 2,4-dinitro-I-dinitroflurobenzene (DNFB) was used in this study. Concanavalin A (Con A)-induced splenocytes proliferation was measured by the MTT colorimetric method and interleukin-2 (IL-2) activity was measured by testing its ability to support ConA-induced mice splenocytes proliferation by MTT method. Interleukin-4 (IL-4) and transforming growth factor -beta 1 (TGF-β 1) levels were determined by ELISA. T lymphocytes subsets were measured by flow cytometry. Results Similar as the control drug 4-acetylaminophenylacetic acid (actarit or Acta) (120 mg·kg -1), GCS (240 mg·kg -1) could inhibit the thymus index and spleen index in CHS mice. GCS( 60,120,240 mg·kg -1) could inhibit the ear swelling of CHS mice and could inhibit the splenocytes proliferation induced by ConA. GCS (240 mg·kg -1)decreased CD4 +CD8 +T lymphocytes subsets ratio and resumed the CD4 +CD8 -subsets ratio in CHS mice;GCS(240,60 mg·kg -1) resumed the CD4 -CD8 -subsets ratio in CHS mice. GCS also decreased the TGF-β 1 and IL-2 levels and increased the IL-4 levels in mice thymus with CHS. Conclusion GCS could inhibit mice CHS reaction and resume the balance of T lymphocytes subsets in mice thymus with CHS, and also could modulate the cytokines production by CD4 + T lymphocytes of CHS mice.

关 键 词：木瓜总苷 接触性超敏反应 T淋巴细胞亚型 CD4^+ CD8^+ 

分 类 号：R-332[医药卫生] R282.71