膜引导齿槽突裂骨缺损区组织再生的实验动物研究  

Experimental study on bioactive membrane for guided bone regeneration in alveolar cleft

作　　者：包崇云[1] 李萍[1] 田卫东[1] 李声伟[1] 

机构地区：[1]四川大学华西口腔医学院,610041

出　　处：《实用口腔医学杂志》2004年第5期535-538,共4页Journal of Practical Stomatology

基　　金：四川省科技厅重点科研基金项目 (G990 0 4 )资助

摘　　要：目的 :评价膜引导齿槽突裂骨再生的效果。方法 :全麻下 ,分别在每组 2只兔双上颌形成人工齿槽突裂 ,随机作 :①聚乳酸 (PLA) +胶原 +rhBMP 2膜覆盖 ;②PLA膜覆盖 ;③胶原膜覆盖 ;④空白对照。分别于术后 2周 ,1、2、3、6月处死动物切取标本 ,摄X线片 ,组织学观察 ,酶组化分析。结果 :膜覆盖骨裂 ,新生骨外形良好 ,骨改建成熟早 ,特别是PLA +胶原 +rhBMP 2膜组 ,可持续控释rhBMP 2并提高骨缺损区浓度 ;空白对照组 ,生成骨质量、外形不良。结论 :引导组织再生膜具有促进齿槽突裂骨修复的作用。Objective: To evaluate the effect of barrier m em brane on guided bone regeneration(GBR) in alveolar cleft. Methods: The alveolar cleft of animal model was established in the maxilla in 10 rabbi ts. The artificial alveolar clefts were treated immediately with following mater ials respectively: (1) PLA+collagen+rhBMP-2 membrane, (2)PLA membrane, (3)colla ge membrane, (4) blank control. All the membranes were fixed by PLA pin and mini -titanium pin. The animals were sacrificed 2 weeks, 1,2,3 and 6 months after su rgery respectively. Samples were studied through macroscopic observation, X-rad iography, histomorphology and enzyme histochemistry analysis. Results: In the clefts covered with the 3 kinds of membrane more bone fromation wa s observed. The compound membrane of PLA+collagen+rhBMP-2 showed the best bone regeneration effects. Conclusion: The GBR technique can be used to repair the bone defect of alveolar cleft.

关 键 词：引导组织再生 齿槽突裂 膜材料 实验动物 

分 类 号：R782.21[医药卫生—口腔医学]