多西紫杉醇对腺样囊性癌SACC-83细胞增殖的影响  

Docetaxel inhibites the proliferation of adenoid cystic carcinoma SACC-83 cells of salivary gland

作　　者：穆云静 吴军正[2] 贾永庆[3] 郭富平[2] 

机构地区：[1]解放军总参第一门诊部,100034 [2]第四军医大学口腔医学院 [3]解放军乌鲁木齐总医院

出　　处：《实用口腔医学杂志》2004年第5期607-609,共3页Journal of Practical Stomatology

摘　　要：目的 :研究多西紫杉醇对涎腺腺样囊性癌SACC 83细胞增殖能力的影响。方法 :用细胞计数法、软琼脂克隆形成法、流式细胞术等研究药物对细胞增殖的抑制作用。结果 :多西紫杉醇对SACC 83细胞具有浓度依赖性和时间依赖性生长抑制作用 ,药物作用 2 4、48、72h的IC3 0 (nmol/L)分别为 1.3 9、1.2 6、0 .47,IC50(nmol/L)分别为 13 .0 2、3 .3 4、1.2 6,相对抗肿瘤活性 (RAA)值分别为 3 3 0、12 98、3 42 6;药物作用于细胞 72h后 ,对照组G1、S、G2 期细胞 ( % )为 73 .8、19.8和 6.4,IC3 0 组为 65 .0、2 9.5和 5 .5 ,IC50 组为 5 7.6、42 .4和 0 ;对照组、IC3 0 组、IC50 组克隆形成率 ( % )分别为 3 6.0± 0 .5、8.3± 2 .5和 0 .5± 0 .3。结论 :多西紫杉醇对腺样囊性癌ACC细胞增殖有明显的抑制作用。Objective:To study the effects of Docetaxel o n the proliferation of adenoid cystic carcinoma SACC-83 cells of salivary gland. Methods:The inhibitory effects of Docetaxel on the proliferatio n of SACC-83 cells were investigated with cell counting, soft agar clonogenic a ssay, and flow cytometry. Results:With the exposure time of 24, 48 or 72 h the IC 30(nmol/L) of Docetaxel was 1.39,1.26 and 0.47, the I C 50(nmol/L) 13.02, 3.34 and 1.26 respectively; the relative antitumor acti vity (RAA) of the drug against SACC-83 cells was 330, 1 289 and 3 426 respectiv ely. After the cells had been treated for 72 h, the percentages of G 1, S and G 2 phase cells in the cell cycle in the control group were 73.8,19.8 and 6.4, in IC 30 group 65.0, 29.5 and 5.5, in IC 50 group 57.6,42.4 and 0, res pectively. The clonogenesity (%) in control, IC 30 and IC 50 groups we re 36.0±0.5,8.3±2.5 and 0.5±0.3 respectively. Conclusion:Doc etaxel may inhibit the proliferation of SACC-83 cells in a dose and time depend ent way.

关 键 词：涎腺 腺样囊性癌 多西紫杉醇 

分 类 号：R739.8[医药卫生—肿瘤]