转录调节因子Ets-1在结直肠癌组织中的表达及其临床意义  

Expression of transcription factor Ets-1 in colorectal cancer: correlation with angiogenesis and progression

作　　者：方开锋[1] 冯贤松[1] 吴河水[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院急诊外科,430022

出　　处：《消化外科》2004年第5期343-346,共4页Journal of Digestive Surgery

摘　　要：目的　研究转录调节因子Ets 1在结直肠癌组织中的表达规律与特点 ,探讨其与肿瘤血管生成和肿瘤侵犯、转移等肿瘤进展的生物学行为的关系。方法　取我院从 2 0 0 1年 9月至 2 0 0 2年 11月间住院手术切除的结直肠癌标本 4 0例 ,同时取 5例正常结直肠组织和 3例结直肠息肉作对照。采用免疫组化技术链霉素抗生物素蛋白 过氧化物酶连接法 (S P法 ) ,检测转录调节因子Ets 1和CD34在它们中的表达特性 ,并结合临床病理参数进行综合分析。结果　正常结直肠组织和结直肠息肉几乎不表达Ets 1,结直肠癌细胞高表达Ets 1;4 0例结直肠癌组织中 ,Ets 1阳性表达及MVD均值显著高于正常结直肠组织和结直肠息肉 ,P <0 .0 1;Ets 1阳性表达组MVD值明显高于阴性组 ,P <0 .0 5 ;Ets 1、CD34在淋巴结转移组、肝转移组、Dukes分期的C期和D期的表达分别显著高于无淋巴结转移组、无肝转移组、Dukes分期的A期和B期 ,P <0 .0 1。结论　 (1)Ets 1促进结直肠癌血管生成 ,并参与肿瘤浸润和淋巴道转移 ,与肝转移有关 ,可作为监测结直肠癌预后的重要指标 ;(2 )检测Ets 1的表达 ,可作为判定结直肠癌血管形成、浸润转移等生物学行为的重要指标。Objective To investigate the expression of transcription factor Ets-1 in colorectal carcinoma and study its correlation with angiogenesis, tumor infiltration and metastasis. Methods Tissue samples were collected from 40 patients who underwent surgery in our hospital from September 2001 to November 2002. 3 colorectal polyps and 5 normal colorectal tissues were used as control. The expression of Ets-1 and CD34 was detected in these samples by immunohistochemistry analylis, and the clinical pathological parameters were analyzed. Results Ets-1 protein was not expressed in normal colorectal tissues and colorectal polyps, but was overexpressed in colorectal carcinoma cells. Positive expression of Ets-1 and the mean MVD value in carcinoma tissues were significantly higher than those in the normal tissues and colorectal polyps (P<0.01). MVD value in the Ets-1 positive group was significantly higher than that in Ets-1 negative group (P<0.05). Values of Ets-1 and MVD in patients with lymph node metastasis, liver metastasis and Dukes C & D stage were markedly higher than those in patients without lymph node metastasis, liver metastasis and patients with Dukes A & B stage (P<0.01). Conclusions (1) Ets-1 can promote angiogenesis in colorectal carcinoma, and play a role in tumor infiltration and metastasis. Expression of can serve as an important index in monitoring the prognosis of colorectal carcinoma. (2) The expression of Ets-1 can be an important indicator for determining the angiogenesis and infiltration of colorectal carcinoma.

关 键 词：转录调节因子 ETS-1 结直肠肿瘤 血管生成 

分 类 号：R735.34[医药卫生—肿瘤]