多不饱和脂肪酸对谷氨酸诱导的大鼠海马神经细胞损伤的双重影响(英文)  

Dual Effects of Polyunsaturated Fatty Acids on Glutamate-Induced Neurotoxicity in Cultured Rat Hippocampal Neurons

作　　者：王新[1] 薛任皓[1] 饶相君[1] 徐俊[1] 刘志礼[1] 

机构地区：[1]南京大学生命科学学院,南京210093

出　　处：《南京大学学报（自然科学版）》2004年第5期598-606,共9页Journal of Nanjing University（Natural Science）

摘　　要：通过测定原代培养的谷氨酸诱导的受损海马神经的细胞存活率,谷胱甘肽过氧化物酶活性,以及谷胱甘肽和一氧化氮的水平,研究多不饱和脂肪酸,如二十二碳六烯酸(docosahexaenoic acid,DHA)、花生四烯酸(arachidonic acid,AA),对大鼠海马神经细胞损伤的影响。海马神经元用神经特异性烯醇化酶进行鉴定。研究发现:(1)DHA 5～50 mg/L能有效的对抗谷氨酸诱导的海马神经细胞的损害作用。表现在细胞存活率、谷胱甘肽过氧化物酶的活性较谷氨酸处理组明显提高,NO水平降低,谷胱甘肽含量与对照组相比变化不大。(2)5 mg/L AA对损伤没有影响,而 30和 50 mg/L AA对损伤具有加重作用,其细胞存活率、GSH- Px活性、GSH水平较谷氨酸处理组明显降低。结果表明,多不饱和脂肪酸对谷氨酸诱导的神经细胞损伤具有双重作用。This study investgated the effects of polyunsaturated fatty acids (PUFAs), such as docosahexaenoic acid (DHA) and arachidonic acid (AA), on glutamate-induced neurotoxicity in cultured rat hippocampal neurons. Detected were the cell viability, the activity of glutathione peroxidase (GSH-Px), and the levels of cellular glutathione (GSH) and nitric oxide (NO). Neurons were identified in use of an antibody raised against neuron specific enolase (NSE). DHA at 5～50 mg/L markedly increased the cell viability, inhibited NO production, and increased the activity of GSH-Px. In addition, no difference occurred in the level of GSH when compared with the control. Five mg/L AA neither alleviated nor aggravated the cytotoxicity, while 30, 50 mg/L AA significantly decreased the survivability, activity of GSH-Px, and the level of GSH, compared with glutamate-exposed cultures. These results suggested that PUFAs have a dual effect on glutamate- induced neurotoxicity.

关 键 词：多不饱和脂肪酸 海马神经元 神经特异性烯醇化酶 谷氨酸神经毒 谷胱甘肽 谷胱甘肽过氧化物酶 一氧化氮 

分 类 号：R963[医药卫生—微生物与生化药学]