邻苯二甲醛-硫酸比色法测定稀奶油中胆固醇含量  

Determination of cholesterol content in cream by O-phthalaldehyde sulphuric acid colorimetry

作　　者：耿倩[1] 生庆海[1] 顾瑞霞[2] 

机构地区：[1]石家庄三鹿集团股份有限公司,河北石家庄050071 [2]扬州大学乳品研究所,江苏扬州225009

出　　处：《中国乳品工业》2004年第9期37-38,共2页China Dairy Industry

基　　金：国家"十五"重大科技攻关奶业专项资助(2002BA518A5)

摘　　要：为建立稀奶油中胆固醇含量的测定方法,采用邻苯二甲醛-硫酸比色法测定稀奶油中胆固醇含量。结果表明,胆固醇在553nm处有最大吸收峰,线性范围在1.00～1.73mg/g,平均回收率为95.07%(n=3,RSD=4.33%)。该方法快速准确,方法稳定,重复性好。Aim to establish a simple and accurate method for the determinatio n of cholesterol in cream. Method O-phthalaldehyde sulphuric acid colorimetry was used. Restult max=553 nm,and standard curve was liner in the concentration rang e 1.00～1.73 mg/g,the average recovery was 95.07%,RSD=3.99%. Conclusion The mo thod was simple ,rapid and reliable.

关 键 词：稀奶油 胆固醇 邻苯二甲醛-硫酸比色法 含量测定 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]