盐度、pH变化对凡纳滨对虾鳃丝Na^+-K^+-ATPase活力的影响  

The Effect of Salinity and pH Changes on Gill Na^+-K^+-ATPase Activity of Litopenaeus vannamei

作　　者：潘鲁青[1] 刘志[1] 姜令绪[1] 

机构地区：[1]中国海洋大学海水养殖教育部重点实验室,山东青岛266003

出　　处：《中国海洋大学学报（自然科学版）》2004年第5期787-790,共4页Periodical of Ocean University of China

基　　金：山东省科技兴海项目"低盐度地下卤水凡纳滨对虾养殖技术的开发研究"(2 0 0 1 3 6)资助

摘　　要：本文研究了盐度、pH变化对凡纳滨对虾 (Litopenaeusvannamei)鳃丝Na+ K+ ATPase活力的影响。结果表明 :盐度、pH变化对凡纳滨对虾鳃丝Na+ K+ ATPase活力的影响显著 (F >F0 .0 1)。在盐度变化 (3 0→ 5 ) 3d内 ,各处理组鳃丝Na+ K+ ATPase活力随着处理时间的增加逐渐升高 ;至 3～ 15d时 ,不同盐度下鳃丝Na+ K+ ATPase活力趋于稳定 ,与对照组相比酶活力明显升高 (F >F0 .0 5) ,而且盐度越低酶活力越大。在 pH变化 (7.0← 8.0→ 9.5 ) 3d内 ,各处理组鳃丝Na+ K+ ATPase活力随着处理时间的增加呈峰值变化 ,表现为向低pH和向高 pH变化分别于 9h和 12h达到最大值 ,至 3d时均恢复正常 ;在pH变化 3～ 12d内 ,不同 pH环境下鳃丝Na+ K+ ATPase活力无明显差异 (F <F0 .0 5)。This paper studies the influence of salinity and pH on gill Na+-K+-ATPase activity of Litopenaeus vannamei. The results indicate that salinity and pH changes have a significant effect on gill Na+-K+-Atpase activity of Litopenaeus vannamei (F>F 0.01). During the first 3 days of the salinity treating, the gill Na+-K+-ATPase activity of the shrimp in all treatments increased gradually with the sampling time, and the enzyme activity in every treatment tended to be stable in the period from the third to fifteenth day and were markedly high as compared to the control treatments(F>F 0.05), meanwhile the salinity lowered as the enzyme activity increased. During the first 3 days of the pH changes, the gill Na+-K+-ATPase activity of all treatments changed in curved loci, and the peaks appeared at 9th hour and 12th hour separately when pH value of assay groups decreased or increased compared with the controls. During the period from the third to twelfth day, gill Na+-K+-ATpase activity of all treatments tended to be stable and had no significant differences (F<F 0.05).

关 键 词：盐度 PH 凡纳滨对虾 鳃丝 NA^+-K^+-ATPASE 

分 类 号：X171.1[环境科学与工程—环境科学] S968.22[农业科学—水产养殖]