柠檬酸钠对枯草杆菌生长代谢及肌苷积累的影响  

Effect of Sodium Citrate on The Growth Metabolism and Inosine Accumulation by Bacillus subtilis

作　　者：刘新星[1] 陈双喜[1] 储炬[1] 庄英萍[1] 张嗣良[1] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

出　　处：《微生物学报》2004年第5期627-630,共4页Acta Microbiologica Sinica

基　　金：上海市曙光计划资助项目 ( 0 1SG2 8)~~

摘　　要：研究柠檬酸钠对枯草杆菌生长代谢及产苷的影响 ,在基础料中添加浓度为 0 2g L的柠檬酸钠 ,肌苷产量提高 18% ,肌苷对葡萄糖得率增加 38%。通过分析糖代谢途径中关键酶的酶活 ,结果表明添加柠檬酸钠改变了一些关键酶的活力 ,可降低糖酵解途径中 6_磷酸果糖激酶和丙酮酸激酶的活力 ,从而减弱了糖酵解途径的通量。The effects of sodium citrate on the growth metabolism and inosine accumulation by Bacillus subtillus were studied in this paper.When 0.2% sodium citrate was added into the basal medium,the inosine yield and the yield coefficient of inosine to glucose in the broth were increased 18% and 38% respectively.The analysis on the activities of some key enzymes during inosine fermentation revealed that the supplement of sodium citrate had profound effect on the activity of several key enzymes. The activities of Phosphofructokinase and Pyruvate Kinase were decreased remarkably,the flux of glycolysis pathway was thus cut down as well.

关 键 词：柠檬酸钠 枯草杆菌 生长 代谢 肌苷 丙酮酸激酶 糖酵解通量 

分 类 号：Q935[生物学—微生物学]