桃中两个MADS box基因的克隆与表达分析  

Cloning and Characterization of Two MADS Box Genes from Peach( Prunus persica )

作　　者：吴凡[1] 徐勇[1] 常凤启[1] 李学东[2] 马荣才[1] 

机构地区：[1]北京农业生物技术研究中心,北京100089 [2]首都师范大学生命科学学院,北京100037

出　　处：《Acta Genetica Sinica》2004年第9期908-918,共11页

基　　金：国家自然科学基金资助 (编号 :3 0 170 64 9)~~

摘　　要：为研究李属 (Prunussp .)果树生殖调控的相关基因 ,对国际公共数据库中的李属植物的EST(expressedse quencetags)序列进行了电子拼接 ,获得了 8个MADSbox基因的cDNA序列 ,并利用PCR技术从桃中克隆出其中的两个cDNA ,分别命名为PpMADS4和PpMADS6 ,在GenBank中的登录号为AY70 5 972和AY70 5 973。PpMADS4基因长85 0bp ,包含一个 732bp的开放阅读框 ,编码 2 4 3个氨基酸。PpMADS6基因长 1190bp ,包含 1个 76 8bp的开放阅读框 ,编码 2 5 6个氨基酸。PpMADS4和PpMADS6在序列上分别与拟南芥中的AGAMOUS基因和矮牵牛中的PFG基因高度同源。RT PCR分析表明 ,PpMADS4基因在桃的花瓣、心皮、果实及果仁中表达 ,应属于控制花器官发育的C类MADSbox基因。PpMADS6基因在桃的叶、萼片、花瓣、心皮及果实中表达 ,应属于调控植物由营养生长向生殖生长过渡的A类MADSbox基因。With the aim of finding genes involved in the floral transition of Prunus species( Prunus sp.),the EST (expressed sequence tags) sequences were extracted from the public databases.Eight MADS box genes’ cDNAs were obtained.Two of them, PpMADS4 and PpMADS6 (The accession numbers in GenBank are AY705972 and AY705973),were cloned from peach ( Prunus persica ).The full length cDNA of PpMADS4 is 850 bp long.It contains an open reading frame of 732 bp,coding for a polypeptide of 243 amino acids.The full length cDNA of PpMADS6 is 1 190 bp long.It contains an open reading frame of 768 bp coding for a polypeptide of 256 amino acids. PpMADS4 closely resembles the Arabidopsis AGAMOUS gene.It is an AGAMOUS -like C class MADS box gene,and it expresses in petal,carpel,fruit and nutlet as demonstrated by RT-PCR analysis. PpMADS6 is likely to be the peach orthologue of the Petunia PFG genes and it is an A class MADS box gene.It has been shown with RT-PCR that it expresses in leaf,sepal,petal,carpel and fruit.It may be involved in the transition from the juvenile to the adult stage.

关 键 词：桃 花发育 MADS BOX 幼年期 成熟期 

分 类 号：S662.1[农业科学—果树学]