新疆3种雅罗鱼线粒体DNA控制区序列的差异和系统进化关系  

Divergence and Systematical Evolution of Three Leuciscus Species in Xinjiang Based on Mitochondrial DNA Control Region Sequences

作　　者：胡文革[1] 段子渊[2] 王金富[3] 盛金良[3] 马润林[2] 

机构地区：[1]石河子大学生物工程学院,石河子832003 [2]中国科学院遗传与发育生物学研究所,北京100101 [3]石河子大学动物科技学院,石河子832003

出　　处：《Acta Genetica Sinica》2004年第9期970-975,共6页

基　　金：石河子大学自然科学基金项目资助 (编号 :80 70 82 2 5 3 2 )~~

摘　　要：对分布在新疆的准噶尔雅罗鱼 (Leuciscusmerzbacheri)、贝加尔雅罗鱼 (Leuciscusleuciscusbaicalensis)和高体雅罗鱼 (Leuciscusidus) 3个鱼种共 2 4尾个体的线粒体DNAD loop控制区核苷酸序列进行了测定 ,获得 2 4条长度为 6 6 7～ 6 6 9bp的同源基因序列。 3种雅罗鱼之间的序列差异在 6 39%～ 9 89%之间 ,贝加尔雅罗鱼与高体雅罗鱼种间序列同源性高 ,变异程度小 ;贝加尔雅罗鱼与准噶尔雅罗鱼种间序列同源性最低 ,变异程度最大。所采集的贝加尔雅罗鱼两个地理群体 (赛里木湖和额尔齐斯河 )内mtDNA的平均核苷酸碱基序列差异为 1 0 7%和 1 0 8% ;两群体间的序列差异为 1 0 7% ,显示两个地理群体间无明显分化。DNA序列数据显示 ,这 3种鱼类线粒体DNAD loop序列变异丰富 ,2 4尾个体呈现独自的单倍型。同源基因序列平均含AT碱基 6 4 1% ,GC碱基 35 9% ,显示准噶尔雅罗鱼、贝加尔雅罗鱼、高体雅罗鱼的线粒体DNAD loop区核苷酸组成的不均一性。分子系统树提示 ,贝加尔雅罗鱼与高体雅罗鱼亲缘关系较近 ,准噶尔雅罗鱼是 3种雅罗鱼中较古老的鱼种。Nucleotide sequence of fish mitochondria DNA (mtDNA) D-loop region from Leuciscus leuciscus baicalensis,L. merzbacheri ,and L.idus in Xinjiang,China,were examined by sequencing 667～669 bp length of homological fragments in the D-loop from 24 individuals of the three fish species. DNA divergence ranged from 6.39%～9.89% among the three fish species in the genius of Leuciscus cuvier .The sequence similarity is high and the variation is low between L. Leuciscus baicalensis and L. idus . In contract,the genetic distance is larger between L.Leuciscus baicalensis and L.merzbacheri . The average nucleotide variation within each of the two geographical populations (Sailimu lake and E’erqis river) of L.leuciscus baicalensis is 1.07% and 1.08%,respectively,and such variation is 1.07% between the two populations. These results demonstrate that the two geographic populations of L.leuciscus baicalensis do not appear to have significant genetic differentiation. Sequencing data showed the existence of sufficient genetic variations among three species of fish,as illustrated by distinct haplotypes for each species. The phylogenetic trees built with MEGA1.02 pointed out that the relationship between L.leuciscus baicalensis and L.idus is close,and L.merzbacheri is ancient among three Leuciscus.

关 键 词：雅罗鱼 线粒体DNA D—loop控制区 系统进化 

分 类 号：Q953[生物学—动物学]