超临界流体CO_2萃取中药有效成分的抗氧化性的分光光度法研究  

Spectrophotomatric Determination of Antioxidative Activity of Extractives of Chinese Traditional Medicine by Supercritical Fluid CO_2 Extraction Technology

作　　者：张立伟[1] 王金山[1] 江崇球[1] 

机构地区：[1]山东师范大学化学系,山东济南250014

出　　处：《光谱学与光谱分析》2004年第9期1103-1105,共3页Spectroscopy and Spectral Analysis

摘　　要：利用超临界流体二氧化碳萃取技术提取中药虎杖、大黄、炒白术中黄酮类化合物。在 pH 8 2的HCl tris缓冲溶液中利用邻苯三酚自氧化法测定提取物对超氧阴离子自由基的消除作用。用茜素紫作显色剂的分光光度法在pH 9 0的HCl tris缓冲体系中测定中药提取物对Co2 + +H2 O2 体系所产生的羟基自由基的消除作用 ,并对实验条件进行了探讨。实验结果表明 ,3种中药提取物对自由基都有消除作用 。Applying supercritical CO2 fluid extraction technology, flavonoids were extracted from Chinese traditional medicine asarum, heterotropoides, atractylodes macrocephala, and rheum palmatun. Using the method of autoxidation pyrogallol (known as 325 nm method), the superoxide radical scavenging effect of the extraction was carried out in the buffer solution of HCl-tris(pH 8.2). With spectrophotometry, hydroxyl radical created by the system Co2+ + H2O2 in the reaction like Fenton reaction was eliminated by alizarin violet as the color developing agent in the buffer solution HCl-tris (pH 9.0) and the reaction condition was investigated. Result showed that these extractions are elimination agent for these radicals. Asarum heterotropoides is the best of the three.

关 键 词：中药 有效成分 抗氧化性 分光光度法 超临界流体二氧化碳萃取 

分 类 号：R284.2[医药卫生—中药学]