TNP-470对肺癌细胞诱导的血管内皮细胞增殖的抑制作用  

Inhibition of TNP-470 on proliferation of vascular endothelial cells induced by pulmonary cancer cells

作　　者：吴晓娟[1] 杨向红[1] 

机构地区：[1]中国医科大学基础医学院实验病理研究室,辽宁沈阳110001

出　　处：《中国医科大学学报》2004年第5期405-407,共3页Journal of China Medical University

摘　　要：目的 :应用体外细胞培养法探讨O (氯乙酰 氨甲酰基 )烟曲霉素 (TNP 4 70 )对肺癌细胞诱导的血管内皮细胞增殖的影响及其可能机制。方法 :免疫细胞化学方法检测血管内皮细胞增殖细胞核抗原 (PCNA)、胚胎肝激酶 1(Flk 1)、纤维细胞生长因子受体 (FGFR)的表达。结果 :TNP 4 70以剂量依赖方式抑制肺癌细胞条件培养液或细胞粉碎液诱导的血管内皮细胞PCNA ,Flk 1,FGFR的表达 ,与对照组比较差异均显著 (P<0 0 5 ) 。结论 :TNP 4 70能够相对特异地抑制血管内皮细胞增殖 ,可能与通过降低内皮细胞表面Flk 1,FGFR的表达 。Objective: To discuss the influence and poss ible mechanism of TNP-470 on the cultured cell in vitro. Methods: The expressions of proliferating cell nuclear gene (PCNA), fetal liver kina se 1 (Flk-1), fibroblast growth factor receptor (FGFR) of endothelial cells w ere tested by using immunocytochemical analysis, positive cell-count, and image analysis. Results: The TNP-470 inhibited the expressions of P CNA, Flk-1, and FGFR of human umbilical vein endothelial cell induced by pulmon ary cancer cell dose-dependently compared with conditional group (P<0.05). Conclusion: The TNP-470 reduced the proliferation of endothe lial cells mediated by receptor tyrosine kinases(RTKs) pathway by weakening the expressions of Flk-1 and FGFR of endothelial cells.

关 键 词：O-(氯乙酰-氨甲酰基)烟曲霉素 人脐静脉内皮细胞 肺癌细胞 

分 类 号：R730.5[医药卫生—肿瘤]