蛇毒神经生长因子抗肿瘤体外实验研究  

In vitro experimental study of the antitumor potency of snake venom nerve growth factor

作　　者：孙晋民[1] 张蕾[2] 姜丽[3] 郑华川[1] 王维琴[3] 辛彦[1] 郝文学[4] 

机构地区：[1]中国医科大学附属第一医院肿瘤研究所第四研究室,辽宁沈阳110001 [2]中国医科大学附属第一医院肿瘤研究所第一研究室,辽宁沈阳110001 [3]中国医科大学附属第一医院肿瘤研究所基础医学院细胞生物学教研室,辽宁沈阳110001 [4]老年病防治研究中心

出　　处：《中国医科大学学报》2004年第5期408-410,共3页Journal of China Medical University

基　　金：国家自然科学基金资助项目 ( 3 0 0 70 845 )

摘　　要：目的 :探讨蛇毒神经生长因子 (NGF)对人肿瘤细胞增殖的抑制作用。方法 :采用噻唑蓝 (MTT)法、台盼蓝拒染法和集落形成作为观察指标 ,以NGF 5 ,10 ,15 ,2 0 μg /ml处理 4种人肿瘤细胞 (人白血病细胞株K 5 6 2、人胰癌细胞株JF 30 5 ,人肺癌细胞株AGZY 83a和人胃癌细胞株MG 80 3) ,选用不同作用时间 ,分别观察NGF对上述细胞的影响。阿霉素 (Adr)为阳性对照药。结果 :NGF对 4种人肿瘤细胞均有细胞毒性作用 ,K 5 6 2和JF 30 5比后两者显示出更好的量效关系 (P均 <0 .0 5 )。NGF处理 2 4 ,4 8和 96h后K 5 6 2和JF 30 5细胞的生长均受到抑制 (P <0 .0 5 ,P <0 .0 1)。并有显著的剂量效应关系。处理JF 30 5细胞 2 4 ,4 8和 96h ,NGF的半抑制浓度 (IC50 ) ,时间 效应关系亦明显 ;NGF对K 5 6 2细胞的生长抑制作用以 4 8h为显著。NGF还可显著抑制JF 30 5细胞的集落形成 ,并有明显的量效关系 (P <0 .0 1) ,其IC50 为 12 .86 μg/ml。 结论 :NGF对 4种人肿瘤细胞均具有细胞毒性和生长抑制作用 ,对K 5 6 2、JF 30Objective: To observe the inhibitory effects of nerve growth factor(NGF) on human tumor cell lines. Methods: The MTT colorimetric method, trypan blue exclusion,and colony formation was u sed. Adr used as positive control. Four kinds of human tumor cell lines, K 562,J F 305, AGZY-83a and MG-803 were treated with NGF at different final concentrat ions of 5,10,15, and 20 μg/ml. The effects of NGF on the cells mentioned above were observed at different intervals after the cell were exposed to NGF. Results:NGF had obvious cytotoxicity on these cell lines. There were obvious dose-response relationship in test of K 562 and JF 305 cells (P< 0.05). K 562 and JF 305 were exposed to NGF at different intervals of 24,48, and 96 h, the growth of both cell lines was inhibited obviously and the dose-r esponse relationships were showed distinctively. At 24, 48, and 96 h intervals a fter JF 305 cells were exposed to NGF,there was obvious time-reponse relationsh ip. The inhibitory effect of NGF on the growth of K 562 reached peak at 48h time point. NGF also possessed inhibitory effect on colony formation of JF 305 cells with satisfied dose-response relationship (P<0.01)and the IC 50 was 12.83 μg/ml. Conclusion:NGF possesses distinct cytotoxicity and growth inhibitory effect on the four kind of human tumor cells especially o n K 562 and JF 305

关 键 词：蛇毒神经生长因子 肿瘤细胞系 抗肿瘤作用 

分 类 号：R730.3[医药卫生—肿瘤]