纤维化及正常肝组织中血管紧张素Ⅱ1型受体mRNA的表达  

Expressions of angiotensin II type1receptor mRNA in fibrotic and normal liver tissues

作　　者：王卫卫[1] 杨希山[1] 吴平生[2] 王捷[3] 张宏斌[3] 李旭[2] 郑文岭[3] 

机构地区：[1]第一军医大学南方医院全军消化内科研究所,广东广州510515 [2]第一军医大学南方医院心血管科,广东广州510515 [3]广州军区广州总医院医学实验科,广东广州510010

出　　处：《第一军医大学学报》2003年第5期477-478,482,共3页Journal of First Military Medical University

基　　金：国家自然科学基金(30270610)~~

摘　　要：目的探讨纤维化及正常肝组织中血管紧张素Ⅱ1型受体(AT1R)mRNA的表达情况。方法应用半定量逆转录聚合酶链反应法检测18例肝癌周围纤维化肝组织和12例正常肝脏组织中AT1RmRNA的表达,同时逆转录AT1R特异性片段和内参片段(GAPDH),以二者的积分吸光度比值作为该受体mRNA的相对表达量。结果纤维化肝组织AT1RmRNA的相对表达量明显高于正常肝组织(1.27±0.45vs0.71±0.21,P<0.01)。结论AT1R可能在肝纤维化的发生发展中起一定的作用。Objective To investigate the expressi on profile of angiotensin Ⅱtype 1 receptor (AT1R) mRNA in fibrotic and normal li ver tissues. Methods Semi-quantitative RT-PCR was used to detect the expression of AT1R mRNA in 18 specimens of fibrotic liver tissues adjacent to liver cancer s and 12 normal liver tissue specimens. Specific AT1R product and GAPDH product used as internal control were both amplified by RT-PCR, and the ratio of their integrated optical densities calculated to estimate the relative quantity of AT 1R mRNA expression. Results The relative mRNA expression of AT1R in fibrotic l iver tissues was significantly higher than that of normal liver tissues (1.27±0 .45 vs0.71±0.21, P<0.01). Conclusion AT1R may play an important role in the de velopment of hepatic fibrosis.

关 键 词：组织纤维化 肝脏 Ⅰ型血管紧张素Ⅱ受体 基因表达 逆转录聚合酶链反应 

分 类 号：R575.2[医药卫生—消化系统]