SARS冠状病毒PUMC_2株N蛋白cDNA的克隆、表达和纯化  

Cloning,Expression and Purification of SARS Coronavirus PUMC_2 Strain Nucleocapsid Protein

作　　者：谈昕煜[1] 樊峥[1] 王华瑾[1] 石磊[1] 阴彬[1] 倪安平 秦川 邹柯[1] 沈岩[1] 袁建刚[1] 强伯勤[1] 彭小忠[1] 

机构地区：[1]中国医学科学院中国协和医科大学基础医学研究所医学分子生物学国家重点实验室,北京100005 [2]Department of Clinical Laboratory,PUMC Hospital,CAMS and PUMC,Beijing [3]Institute of Experimental Animal,CAMS and PUMC,Beijing

出　　处：《中国医学科学院学报》2003年第5期504-507,共4页Acta Academiae Medicinae Sinicae

摘　　要：目的原核表达并纯化SARS冠状病毒N蛋白。方法用RT-PCR技术克隆SARS冠状病毒PUMC2株N蛋白全长cDNA,cDNA经序列分析鉴定后,克隆到pET32a表达载体,转化E.coliBL21进行原核表达并纯化出SARS冠状病毒N蛋白。结果实现了SARS冠状病毒N蛋白的表达及纯化。结论用基因重组方法表达的SARS冠状病毒N蛋白可为其进一步功能研究提供条件。To clone,express and purify nucleocapsid protein from SARS coronavirus PUMC 2 strain.Methods According to the published SARS coronavirus genome sequences,the full length cDNA of N protein from SARS coronavirus PUMC 2 strain was cloned by RT-PCR and the cDNA was cloned into the pET32a expression vector.The recombinant N protein was expressed in E.coli BL21(DE3),and purified by Ni 2+ -NTA.Results Prokaryoticly expressed and purified N protein of SARS coronavirus PUMC 2 strain was obtained.Conclusions The SARS coronavirus recombinant N protein obtained by genetic engineering methods can be used for further functional study of SARS coronavirus N protein.

关 键 词：SARS冠状病毒 N蛋白 

分 类 号：Q786[生物学—分子生物学]