土壤中克百威残留检测直接竞争ELISA试剂盒的研制  

Development of the detection direct ELISA kit for carbofuran residue in soil

作　　者：吴慧明[1] 杨挺[1] 朱国念[1] 

机构地区：[1]浙江大学农药与环境毒理研究所,浙江杭州310029

出　　处：《浙江农业大学学报》2003年第6期634-638,共5页

基　　金：浙江省自然科学基金资助项目(301283).

摘　　要：研制了一种用于检测土壤中克百威残留量的直接竞争ELISA(酶联免疫分析技术)试剂盒,研究结果表明:该试剂盒的最低检测限为0.01mg/L,线性检测范围为0.01～10mg/L,4种水样的批内、批间、整体变异系数均低于7.72%,回收率的范围在93.97%～97.92%。试剂盒在4℃或-20℃下至少可保存6个月以上。A ELISA kit based on polyclonal antibody was developed to detect the residue of carbofuran in soil. Linear detection of the kits covered a range from 0.01 mg/L to 10.00 mg/L. The mean within- assay?between-assay and total-assay variability were less than 7.72%. The recoveries of carbofuran added to sample ranged from 93.974% to 97.92%. The validity of kit in 4 ℃ or -20 ℃ was both above six months.

关 键 词：土壤 克百威 残留 ELISA试剂盒 线性检测 

分 类 号：X132[环境科学与工程—环境科学]