用光敏生物素标记HPV16,18DNA探针检测宫颈癌组织核酸相关序列  

Detection of Human Papillomaviruses Type 16, 18 DNA in Cervical Carcinoma Tissue with Photobiotin Labelled Probe

作　　者：张晓燕[1] 谷鸿喜[1] 迟明超[1] 李绍贤[1] 隋丽华[2] 娄阁[2] 关光伟[2] 

机构地区：[1]哈尔滨医科大学微生物学教研室 [2]哈尔滨医科大学三院妇科

出　　处：《哈尔滨医科大学学报》1993年第3期185-187,共3页Journal of Harbin Medical University

摘　　要：本文采用光敏生物素法标记人乳头瘤病毒(HPV)16,18型DNA探针,进行斑点杂交检测宫颈癌组织中HPV16、18DNA相关序列。检测结果为,18例宫项癌组织中5例HPV16杂交阳性(27.78%);14例HPV18杂交阳性(77.8%)。这表明宫颈癌的发生和HPV感染密切相关。The human papillomaviruses type 16, 18 DNA probe were prepared by phtobiotin labelled method and spot hybridization method was used to detect HPV 16, 18 DNA in the tissue of cervical carcinoma. The results indicated that the hybridization positive rate with HPV 16 DNA probe Was 27.78% (5/18) and the positive rate with 18 DNA probe was 77.8% (14/18)。Thus there was a high correlativity between the cervical carcinoma and HPV 16, 18 infection.

关 键 词：基因探针 宫颈癌 斑点杂交 

分 类 号：R737.330.3[医药卫生—肿瘤]