树突状细胞融合在活化抗肿瘤免疫反应中的作用  

Dendritomas plays an important role in the activation of anti-tumor immune responses

作　　者：许峰[1] 王杉[1] 叶颖江[1] 

机构地区：[1]北京大学人民胃肠外科,外科肿瘤实验室100044

出　　处：《中华医学杂志》2004年第9期725-729,共5页National Medical Journal of China

基　　金：教育部科学技术研究重大基金资助项目 (2 0 0 1 171)

摘　　要：目的　探讨树突状细胞 -结肠癌细胞融合在活化T细胞主要组织相容性复合体 (MHC)限制性反应中的作用。方法　使用 5 0 %聚乙二醇将人外周血树突状细胞与结肠癌细胞SW4 80融合 ,用其致敏T淋巴细胞 ,观察T细胞的活化情况。结果　融合细胞与T淋巴细胞混合后 ,CD4 + Th1细胞和CD8+ Tc1细胞的活化率分别为 5 7 4 5 %和 77 86 % ,而对照组无明显活化 ;T细胞的增殖明显高于对照组 (P <0 0 1) ;T细胞分泌的IFN γ明显高于对照组 (P <0 0 1)。结论　树突状 -肿瘤细胞融合策略能有效地活化抗肿瘤MHC Ⅰ类反应和Ⅱ类反应 ,促进T细胞增殖。Objective To investigate the role of dendritomas from fusion of human dendritic cells (DCs)and colon cancer cells in the activation of anti-tumor MHC restricted reaction. Methods Human dendritic cells and T cells were isolated from peripheral blood. Dendritomas were generated by fusing the human peripheral blood dendritic cells with human colon cancer cells of the SW480 line using 50% polyethylene glycol (PEG). T cells were cultured. Dendritomas and DCs were added into the culture respectively. The activation ratio of T lymphocytes was observed by MTT method. ELISA was used to detect the content of interferon-γ (IFN-γ) in the supernatant. Results The activation ratios of CD4 +Th1 and CD8 +Tc1 cells were 57.45% and 77.86 % respectively 24 hours after the T cell were mixed with dendritomas,both significantly higher than those of the controls. The proliferation of T cells mixed with dendritomas was significantly higher than that of the controls ( P <0.01);The IFN-γ secretion in the supernatant of the T cell culture 24 hours after mixing with the dendritomas was significantly higher than that of the control ( P <0.01). Conclusion The strategy of dendritomas effectively activates the anti-tumor MHC-Ⅰ and MHC-Ⅱ reactions and promoted the proliferation of T cells.

关 键 词：树突状细胞 肿瘤 免疫反应 细胞融合 T细胞 胃肠道 

分 类 号：R735.3[医药卫生—肿瘤]