重症急性胰腺炎大鼠血清高迁移率族蛋白-1水平的时相变化及意义  

Serum Levels and Roles of High Mobility Group Box 1 in Severe Acute Pancreatitis Rats

作　　者：杨智勇[1] 王春友[1] 熊炯炘[1] 陶京[1] 许逸卿[1] 刘涛[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院胰腺外科中心,武汉430022

出　　处：《华中科技大学学报（医学版）》2004年第4期466-468,共3页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

摘　　要：目的　观察重症急性胰腺炎 (SAP)大鼠血清肿瘤坏死因子α (TNF α)、白细胞介素 1β (IL 1β)和高迁移率族蛋白 1(HMGB1)水平的时相变化 ,探讨HMGB1在SAP病程中的意义。方法　采用胰管逆行灌注 5 %牛磺胆酸钠的方法复制大鼠SAP模型。随机分为正常对照组 (N组 ,n =8)、假手术组 (Sham组 ,n =8)和重症急性胰腺炎组 (SAP组 ,n =80 )。用酶联免疫吸附试验 (ELISA)检测各组动物血清TNF α、IL 1β水平。用Westernblot法检测血清HMGB1水平。结果　SAP组大鼠血清TNF α和IL 1β水平在建模后迅速升高 ,约在 4～ 6h达高峰 ,之后迅速下降 ,在建模 12h即降至接近正常水平 ,一直维持至 2 4和 4 8h。SAP组大鼠血清HMGB1水平在建模后 12h开始有明显升高 ,至 2 4和 4 8h仍维持在较高水平。结论　HMGB1可能作为晚期炎症因子参与了SAP的全身炎症反应。Objective To observe the serum levels of high mobility group box 1 (HMGB1) in severe acute pancreatitis (SAP) rats.Methods SAP rat model was induced by retrograde injection of artificial bile into the pancreatic duct. Animals were divided randomly into three groups: normal control (n=8), sham operation (n=8), SAP (subdivided into 3, 6, 12, 24, 48 h post-SAP, n=80). TNF-α and IL-1β serum concentrations were measured by ELISA. HMGB1 serum levels were detected by Western blotting. Results The serum concentrations of TNF-α and IL-1β were increased quickly after operation, and reached the peak at 4 or 6 h, but rapidly declined to normal levels at 12 h and maintained to 24 and 48 h. The serum levels of HMGB1 were increased significantly at 12 h, and maintained at high levels up to 48 h after operation.Conclusion HMGB1 seems to act as a late mediator in SAP.

关 键 词：重症急性胰腺炎 大鼠 SAP 血清 高迁移率族蛋白-1 肿瘤坏死因子Α TNF-α 

分 类 号：R576[医药卫生—消化系统]