凋亡素-2配体对胰腺癌细胞PC3和Panc1作用研究  

Apoptosis of the Pancreatic Carcinoma Cell Lines Panc1 and PC3 Induced by Tumor Necrosis Factor-related Apoptosis-inducing Ligand

作　　者：陈鑫[1] 王春友[1] 余康敏[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院胰腺外科中心,武汉430022

出　　处：《华中科技大学学报（医学版）》2004年第4期469-471,共3页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

摘　　要：　目的　了解凋亡素 2配体 (又称肿瘤坏死因子相关凋亡诱导配体 ,TRAIL)对人胰腺癌细胞株Panc1、PC3凋亡作用的影响。方法　采用MTT法、琼脂糖凝胶电泳以及流式细胞仪方法检测TRAIL对Panc1和PC3体外增殖的影响。结果　TRAIL可诱导胰腺癌细胞Panc1和PC3的凋亡 ,并呈时间和浓度依赖性 ,PC3的凋亡率明显高于Panc1。结论　TRAIL可以诱导胰腺癌细胞的凋亡 ,胰腺癌细胞系对TRAIL的敏感性有差异 ,TRAIL可能为胰腺癌的生物学治疗提供一种新途径。Objective To investigate apoptosis of the pancreatic carcinoma cell lines Panc1 and PC3 induced by tumor necrosis factor-related apoptosis-inducing ligand (TRAIL). Methods Apoptosis of pancreatic cancer cells PC3 and Panc1 was detected by MTT assay, agarose gel electrophoresis and flow cytometry. Results Apoptosis of pancreatic carcinoma cell lines Panc1 and PC3 could be induced by TRAIL in a time- and concentration-dependent manner. Conclusion TRAIL can induce apoptosis of pancreatic cancer cell lines PC3 and Panc1.There are some differences in sensitivity to TRAIL-induced apoptosis. TRAIL may provide a new biological therapy on pancreatic carcinoma.

关 键 词：凋亡素-2 配体 胰腺癌 癌细胞PC3 PANC1 TRAIL 肿瘤 

分 类 号：R735.9[医药卫生—肿瘤]