不同因素对小鼠卵母细胞电激活及孤雌发育的影响  

Effects of different factor on electroactivation and parthenogenesis of mouse oocytes

作　　者：马利兵[1] 王强[1] 王凤梅[2] 张涌[1] 

机构地区：[1]西北农林科技大学生物工程研究所,陕西杨凌712100 [2]包头市农牧学校,内蒙古包头014100

出　　处：《西北农林科技大学学报（自然科学版）》2004年第10期66-70,共5页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家"863"高技术研究发展计划项目(2001AA213081)

摘　　要：　研究了卵龄、电脉冲参数、氯化锶、亚胺环己酮(CHX)对小鼠卵母细胞电激活及孤雌发育效果的影响。结果表明,卵龄为17h的小鼠卵母细胞在场强1.0kV/cm,脉冲宽度30μs,2次电脉冲,激活液中含Ca2+、Mg2+和Sr2+的条件下激活后,再于含CHX的培养液中培养,可较好地激活卵母细胞,提高体外孤雌胚的发育率,激活率及桑囊胚率分别可达83.3%和57.5%。This study was designed to evaluate the effect of age,electric pulse parameter,SrCl_2,cycloheximide (CHX) on electroactivation and parthenogenesis of mouse oocytes.The results showed that mouse oocytes which were cultured in CHX-contained CZB medium in vitro after electric activation could obtain higher activated percentage and developmental percentage.Oocytes came from mouse 17 hours after being injected with HCG.Medium of activation contained Ca^(2+),Mg^(2+) and Sr^(2+).The condition of electroactivation was field strength 1.0 kV/cm,pulse duration 30 μs,two times pulses.The percentage of activation and morulae/blastocysts could reach 83.3% and 57.5% respectively.

关 键 词：小鼠 卵母细胞 电激活 孤雌发育 氯化锶 亚胺环已酮 

分 类 号：S865.13[农业科学—野生动物驯养]