小干扰RNA特异性抑制肺肿瘤细胞A549中绿色荧光蛋白基因表达  

Specific suppression of GFP gene expression by siRNA of GFP in lung carcinoma cell A549

作　　者：赵亚力[1] 韩为东[1] 宋海静[1] 李琦[1] 马学斌[1] 郝好杰[1] 

机构地区：[1]解放军总医院基础所分子生物学研究室,北京100853

出　　处：《军医进修学院学报》2004年第4期290-292,共3页Academic Journal of Pla Postgraduate Medical School

基　　金：北京市自然科学基金 ( 70 42 0 5 9)

摘　　要：目的 :探讨绿色荧光蛋白 (greenfluorescenceprotein ,GFP)特异的小干扰RNA (smallinterferenceRNA ,siRNA)对肺肿瘤细胞A5 4 9中的GFP基因表达的抑制作用 ,建立以真核表达载体释放生成特异基因siRNA的方法。方法 :构建mU6启动子驱动的含有GFP特异siRNA的真核表达质粒 ,转染A5 4 9肺肿瘤细胞 ,观察共转染时不同剂量siRNA对GFP的抑制效应。结果 :针对GFP核酸序列设计的特异性siRNAGFP siRNA可有效抑制其表达 ,并当比例为 1∶1时效果最佳 ,而空质粒载体及无关RNAi无此效应。结论 :以mU6为启动子的含特异RNAi的质粒可作为抑制特异基因表达的一种新的技术手段 ,并且为研究基因功能的一条有效途径。Objective:It was designed to investigate whether small interfering RNA (siRNA) of green fluorescence protein (GFP) can inhibit the expression of GFP in lung carcinoma cell A549.Methods:A plasmid included mU6 promoter and siRNA of GFP was designed and constructed. The plasmid was transfected into lung carcinoma cell A549. GFP-siRNA effect of interference to GFP is observed and evaluate in A549 cell. Results: GFP-RNAi can inhibit specific expression of GFP gene in lung carcinoma A549 cell line and mU6 plasmid, no-related-siRNA have no such specific effect.Conclusion: These observations may open a path forward the use of siRNA as a new tool for researching gene function in cancer cell line.

关 键 词：小干扰RNA 肺肿瘤 肿瘤细胞 A549细胞 绿色荧光蛋白 基因表达 

分 类 号：R734.2[医药卫生—肿瘤]