DARPP-32在胃癌中的表达及其意义  

Expression and significance of DARPP-32 in gastric carcinoma

作　　者：王晋[1] 潘阳林[1] 刘娜[1] 郭长存[1] 洪流[1] 樊代明[1] 

机构地区：[1]第四军医大学西京医院消化病研究所,西安710032

出　　处：《中华病理学杂志》2004年第4期350-353,共4页Chinese Journal of Pathology

基　　金：国家自然科学基金重点资助项目(30030140)

摘　　要：目的 探讨DARPP-32蛋白在胃癌组织、细胞系及耐药细胞系中的表达及意义。方法用免疫组织化学SABC法检测96例胃癌组织及57例正常胃黏膜中DARPP-32的表达,用Western蛋白印迹检测胃癌组织及细胞系中DARPP-32的表达。结果 胃腺癌组织中DARPP-32的表达率(92.7％,89/96)显著高于正常胃组织(52.6％,30/57,P<0.05),且DARPP-32表达与胃腺癌的分化、浸润、转移无关(P>0.05)。在胃癌患者的癌组织中DARPP-32和剪切产物t-DARPP均表达,其表达水平显著高于其癌旁组织(t=2.45,P=0.015),并以t-DARPP的表达为主。在人胃癌细胞系中也存在DARPP-32和t-DARPP的表达,而且DARPP-32在SGC7901耐药细胞株SGC7901/VCR和SGC7901/ADR中的表达水平较其亲本细胞明显下调。结论 DARPP-32在胃腺癌组织中的表达增高,DARPP-32参与胃癌的发生,其信号转导通路可能存在于胃上皮细胞,并与胃癌的发生及多药耐药相关。Objective To investigate the significance of DARPP-32 protein expression in gastric carcinoma tissue and cell lines. Methods The expression of DARPP-32 protein in normal gastric mucosa and gastric carcinoma tissue was evaluated by immunohistochemical staining using streptavidin-biotin complex technique. The expression in gastric carcinoma tissue and cell lines was evaluated by Western blotting. Results The expression rate of DARPP-32 protein in gastric adenocarcinoma tissue (92.7% ) was significantly higher than that in normal gastric mucosa (52. 6% , P < 0. 05 ). There was no significant association between DARPP-32 protein expression and degree of tumor differentiation, local invasion and distant metastasis. As compared with adjacent non-carcinomatous gastric mucosa, both DARPP-32 and its truncated isoform t-D ARPP were overexpressed in gastric adenocarcinoma tissue (t=2.45,P=0.015) ; and t-DARPP overexpression was more frequently seen. Expression of DARPP-32 and t-DARPP could also be detected in human gastric cancer cell lines. The expression of DARPP-32 protein was obviously reduced in SGC7901 drug-resistant cell strains. Conclusions DARPP-32 is overexpressed in gastric carcinoma. It may play an important role in gastric carcinogenesis. The underlying signal pathways in neoplastic gastric epithelium may also be related to the multi-drug resistance property of gastric cancer cells.

关 键 词：DARPP-32 胃癌 基因表达 免疫组织化学 检测 

分 类 号：R735.2[医药卫生—肿瘤]