人成釉细胞瘤中pRb和E2F-1表达与端粒酶活性的关系  

Expression of pRb and E2F-1 and telomerase activity in ameloblastoma

作　　者：钟鸣[1] 王洁[1] 张波[2] 侯琳[2] 岳阳丽[1] 李自娟[1] 

机构地区：[1]中国医科大学口腔医学病理科,沈阳110002 [2]北京大学医学部病理系

出　　处：《中华口腔医学杂志》2004年第5期406-409,共4页Chinese Journal of Stomatology

基　　金：辽宁省自然科学基金 (2 0 0 3 2 0 5 7);辽宁省教育厅基金(2 0 12 2 17);沈阳市科委基金(2 0 0 13 8 7)资助项目

摘　　要：目的　研究 pRb、E2F 1和细胞周期素E(cyclinE)在人成釉细胞瘤 (ameloblastoma,AB)中的表达及其与端粒酶 (hTERT)的关系 ,探讨其临床生物学意义。方法　用原位杂交和免疫组化SP法分别检测了AB中 pRb、E2F 1、cyclinE及hTERTmRNA的表达。结果　pRb在AB细胞核中阳性率为2 0 4 % ( 11/ 5 4 )。E2F 1mRNA阳性率为 92 6 % ( 5 0 / 5 4 )。cyclinEmRNA阳性率为 6 6 7% ( 36 / 5 4 )。hTERTmRNA阳性率为 94 4 % ( 5 1/ 5 4 )。伴随AB的复发与恶变 ,hTERT、E2F 1、cyclinE的表达逐渐增高 ,pRb表达丢失。hTERT与E2F 1、cyclinEmRNA之间经Spearman相关分析 ,rs 均为 1 0 0 0 ,P =0 0 0 0 1,呈高度正相关。结论　pRb/E2F 1与AB细胞的增殖、去分化相关 ,端粒酶活性的释放激活与pRb的低表达及E2F 1的高表达可能相关 。Objective To investigate the expression of pRb and E2F 1, and the association between their expression and the activity of telomerase (hTERT) or cyclin E in human ameloblastoma (AB), and to explore the clinical biological characteristics of AB. Methods The expressions of pRb, E2F 1 , cyclin E and hTERT mRNA in human AB were detected by in situ hybridization or immunohistochemistry (SP method). Results The positive expression ratio of pRb in the cell nucleus of AB was 20 4% (11/54). The positive ratio of E2F 1, cyclin E and hTERT mRNA was 92 6% (50/54), 66 7% (36/54) and 94 4% (51/54), respectively. With AB recurrence and malignant transformation , the expression of hTERT, E2F 1, cyclin E was up regulated. hTERT and cyclin E or E2F 1 mRNA had high positive relation (Spearsman′ r s =1 000, P =0 0001). Conclusions The regulatory pathway of Rb /E2F 1 is associated with the cell proliferation and indifferentiation of AB. The activity or release of telomerase may be related to the lower expression of Rb and higher expression of E2F 1, and is up regulated in G 1 late phase by cyclin E.

关 键 词：成釉细胞瘤 PRB E2F-1 端粒酶活性 细胞周期素E 

分 类 号：R739.8[医药卫生—肿瘤]