釉蛋白在大鼠牙胚发育过程中的转录表达  

Enamelin transcriptional expression in developing postnatal rat tooth germ

作　　者：吕平[1] 高学军[1] 贾弘禔[2] 王嘉德[1] 高岩[3] 魏明洁[3] 

机构地区：[1]北京大学口腔医学院牙体牙髓科,100081 [2]北京大学医学部生化与分子生物学教研室 [3]北京大学口腔医学院病理科,100081

出　　处：《中华口腔医学杂志》2004年第5期414-417,共4页Chinese Journal of Stomatology

摘　　要：目的　观察釉蛋白在大鼠牙胚发育过程中的转录表达 ,为进一步研究釉蛋白的生物学功能提供基础。方法　采用原位杂交方法 ,检测出生后 1、3、7、10、14d龄大鼠第一磨牙牙胚中釉蛋白mRNA的表达。结果　大鼠出生后 1～ 10d在成釉细胞和成牙本质细胞中均检测到釉蛋白mRNA的表达。在成釉细胞中的表达随细胞的分化、基质分泌具有规律性 ,1d已有微弱表达 ,3d表达增强 ,7d达到最强 ,10d减弱 ,14d为阴性。釉蛋白mRNA在成牙本质细胞中的表达 1d很微弱 ,14d阴性 ,3～ 10d为持续的中等强度的表达。结论　釉蛋白在成釉细胞中的转录表达从前成釉细胞一直持续到成熟期 ,至牙冠硬组织发育完全时中止。成牙本质细胞也表达釉蛋白基因 。Objective To observe the transcriptional expression of enamelin in developing postnatal rat first mandibular molar germs, for further studies of functions of enamelin in enamel development and mineralization. Methods Tissue slices of first mandibular molar germ of rat 1, 3, 7, 10, 14 days after birth were prepared. The enamelin mRNA expression was identified by in situ hybridization. Results Enamelin mRNA was observed in both ameloblast and odontoblast in 1 10 day old rat postnatal first mandibular molar germs. Enamelin mRNA appeared very weakly at 1st day, and increased through 3rd day, reached the maximum at 7th day, and reduced at 10th day and became negative at 14th day postnatally; while the expression of enamelin mRNA in odontoblast maintained lower from 1st to 10th day and negative at 14th day postnatally. Conclusion Enamelin gene transcriptional expression lasts from preameloblast to maturation ameloblast, which suggests that enamelin may participate in the development of enamel and mantle dentin.

关 键 词：釉蛋白 大鼠 牙胚发育 基因转录 基因表达 生物学功能 原位杂交 

分 类 号：R78[医药卫生—口腔医学]