淫羊藿抑制颌骨骨吸收的体外实验研究  

Effect of Epimedium grandiflorum Morr on osteocalstic bone resorption

作　　者：王晶[1] 于世凤[1] 庞淑珍[1] 

机构地区：[1]北京大学口腔医院口腔病理科,100081

出　　处：《中国骨质疏松杂志》2004年第3期277-279,共3页Chinese Journal of Osteoporosis

基　　金：国家自然科学基金资助项目 ( 3 983 0 43 0 )

摘　　要：从乳兔长骨分离出培养破骨细胞 ,并与人下颌骨骨磨片体外共同培养 ,分别加入不同浓度 (0 ,0 15 ,1 5和 15mg/ml)的淫羊藿注射液 ,倒置相差显微镜观察破骨细胞形成的骨吸收陷窝 ,并对陷窝数目和面积进行图像分析 ,探讨淫羊藿对破骨细胞性颌骨骨吸收的影响。结果显示 ,与对照组相比 ,淫羊藿 3个实验组 (0 15 ,1 5和 15mg/ml)均能有效抑制破骨细胞在下颌骨片上形成的吸收陷窝的数量和面积 ,并且抑制作用呈剂量依赖性递增。Osteoclasts were isolated from long bones of newborn rabbits and cultured on human mandible slices in vitro. Each bone slice was placed in a 24-well culture plate containing various concentrations of Chinese herb Epimedium grandiflorum Morr in α-MEM.The effect of Epimedium grandiflorum Morr on bone resorption lacunae generated by osteoclasts was observed under phase-contrast microscope. The results showed that Epimedium grandiflorum Morr various concentrations(0.15 mg/ml,1.5 mg/ml,15 mg/ml)have significant inhibitory effect, which is increased with concentration of the drug.

关 键 词：淫羊藿 骨吸收 破骨细胞 下颌骨 体外实验研究 抑制 长骨 对照组 共同培养 剂量依赖性 

分 类 号：R782[医药卫生—口腔医学] R681[医药卫生—临床医学]