膳食多不饱和脂肪酸(n-6)/(n-3)不同比率对尾悬吊大鼠骨代谢的影响  

Effects of different (n-6)/(n-3) ratios of polyunsaturted fatty acids on bone metabolism in tail-surpended rats

作　　者：郑冬梅[1] 白树民[2] 黄纪明[2] 李志霞[2] 朱德兵[2] 石阶平[3] 

机构地区：[1]北京航空航天大学研究生院,100083 [2]航天医学工程研究所 [3]中国农业大学食品学院

出　　处：《中国骨质疏松杂志》2004年第3期284-287,共4页Chinese Journal of Osteoporosis

摘　　要：目的　研究多不饱和脂肪酸 (n 6 ) / (n 3)的不同比率对悬吊大鼠骨代谢和组织脂肪酸成分的影响 ,为失重骨丢失的防护提供实验依据。方法　SD大鼠随机分 5组 ,分别为 :I组 :(n 6 ) /(n 3) =7 79、Ⅱ组 :(n 6 ) / (n 3) =3 75、III组 :(n 6 ) / (n 3) =1 4 7、SC组 :悬吊对照组、FAC组 :自由活动对照组。每天上午 9∶0 0灌胃 ,实验期为 2 1d。结果　与SC组相比 ,III组大鼠血清骨钙素含量显著增加 ,股骨生物力学指标明显改善 ;随着 (n 6 ) / (n 3)PUFA比率的降低 ,肝脂中花生四烯酸 (AA 2 0 :4n 6 )含量降低 ,n 3PUFA含量上升。结论　降低膳食 (n 6 ) / (n 3)PUFA比率可改善尾悬吊大鼠的骨代谢和骨结构 ,提高骨生物力学性能。Objective To study the effects of different dietary (n-6)/(n-3) ratios of polyunsaturated fatty acids (PUFA) on bone metabolism and tissue fatty acid composition in rats with simulated weightlessness. Methods Male SD rats were divided into the following groups: the free active control group (FAC),the tail-suspended control group (SC),and the other three tail-suspended groups which were treated with (n-6)/(n-3) ratios of 7.79 (group I), 3.75(group Ⅱ) and 1.47 (group Ⅲ) of fatty with. The duration of experiment lasted for 3 weeks. The influence of the (n-6)/(n-3) PUFA ratios on the bone biomechanical properties, bone biochemical markers , and the tissue fatty acids composition were investigated. Results Group Ⅲ exhibited significantly higher BGP and better femoral biomechanical properties than group SC. With the decrease in the ratio of (n-6)/(n-3) PUFA, the content of arachidonic neid (20:4 n-6) in liver decreased, and the n-3 PUFA increased. Conclusion Decreasing the dietary (n-6)/(n-3) ratio of PUFA improves the bone metabolism and bone structure, and increases bone biomechanical properties.

关 键 词：悬吊 大鼠 骨代谢 膳食 对照组 骨生物力学 含量 比率 骨结构 肝脂 

分 类 号：R681[医药卫生—骨科学] R285.5[医药卫生—外科学]